摘要
目的 :比较聚合酶链反应 -微孔板杂交法 (PCR- MPH )和聚合酶链反应 -聚丙烯酰胺凝胶电泳法 (PCR-PAGE)检测肿瘤组织端粒酶活性的临床价值。 方法 :PCR- MPH以地高辛标记端粒重复片段特异的探针与 PCR变性产物杂交 ,经酶底物显色 ,通过测定吸光度判断结果 ;PCR- PAGE方法直接用聚丙烯酰胺凝胶电泳法分离PCR扩增端粒酶的合成产物 ,经银染色 ,分析端粒酶活性。 结果 :PCR- MPH方法能准确、特异地检测出肿瘤组织的端粒酶活性 ,其灵敏度比 PCR- PAGE法高 10 0倍。 结论 :两种方法均可用于临床检测 ,只是 PCR- MPH方法更简单、方便一些。
Objectives:To detect telomerase activity in various tumor tissues by PCR PAGE and PCR MPH. Methods:We used telomerase PCR MPH,an assay in which a digoxigenin labeled telomerase repeat specific probe was hybridized to the PCR product. After revelation with an anti digoxin AKP conjugates, the amount of hybridized probe was determined by optical reading. Telomerase activity was analyzed by PAGE. Results:Telomerase activity was determined by PCR MPH accurately and specifically in various tumor tissues, its sensitivity was one hundred times higher than that of PCR PAGE. Conclusion:PCR MPH is ideal for analyzing telomerase activity in various tumor tissues. It is simple, easy and suitable for clinical application.
出处
《医学研究生学报》
CAS
2001年第2期112-114,共3页
Journal of Medical Postgraduates
