摘要
目的 :探讨癌基因HER2、mdm 2和C myc扩增与胃癌恶性程度、转移及预后的关系。 方法 :用差异竞争性多聚酶链反应检测胃癌原发灶、癌旁、转移淋巴结及远处脏器转移癌中HER2、mdm 2和C myc的基因变异。结果 :HER2扩增频率在近端胃癌组中明显高于远端胃癌组 (分别是 11/ 13和 5 / 19,P <0 .0 0 5 ) ,侵及浆膜组明显高于未侵及浆膜组 (12 / 18和 4/ 14,P <0 .0 5 )。mdm 2的扩增频率在转移淋巴结中高于胃原发癌 (12 / 2 1和 12 / 32 ,P>0 .0 5 ) ,在 3例淋巴结微转移灶中发现mdm 2扩增。C myc在胃癌原发灶及转移淋巴结中的扩增频率分别是6 / 32和 5 / 2 1,2例远处脏器转移癌中有扩增。结论 :HER2、mdm 2和C myc基因扩增与胃癌的快速生长有关 ,三者联合检测可能为判断胃癌恶性程度、转移及预后提供有价值的信息。
Objective: To determine the relationship between the amplification of the oncogene HER2, mdm 2 and C myc and the malignant degree,metastasis and prognosis of gastric cancer (GC). Methods: Using differential competitive polymerase chain reaction(DC PCR), we detected the amplification of HER2, mdm 2 and C myc in gastric primary carcinoma and metastases foci. Results: There were significantly increased frequencies of the HER2 amplification in the proximal GC group compared with the distal GC group, and in the invaded serosa group compared with no invaded serosa groupe (11/13 vs 5/19, P <0.005; 12/18 vs 4/14, P <0.05). The frequency of mdm 2 amplification was higher in the metastasis lymphnodus than in the primary carcinoma(12/21 and 12/32 respectively). mdm 2 amplification was detected in 3 specimens of lymph micro metastasis foci. The frequencies of the C myc amplification were 6/32 in gastric primary carcinoma, and 5/21 in the metastasis lymphnodus. The C myc amplification was found in two cases of the distant organic metastasis. Conclusion: The activity of the oncogene HER2, mdm 2 and C myc is correlated with the progress of GC. The valuable information may be supplied for judging the malignant degree and metastasis, and predicting the prognosis of GC by detecting these genes together. (J Beijing Med Univ, 2000,32:105 108)
出处
《北京医科大学学报》
CSCD
2000年第2期105-108,共4页
Journal of Peking University(Health Sciences)
基金
国家杰出青年基金!( 3 962 5 2 6)
北京基因诊断实验室项目
关键词
胃肿瘤
遗传学
基因扩增
预后
生物学行为
Stomach neophlasms/genet
Gene amplification
Prognosis
Proto oncogene protein C myc/anal
