摘要
目的 :观察神经生长因子对人牙乳头间充质细胞增殖和细胞周期的影响 ,探讨其在牙胚生长发育过程中的可能作用。方法 :采用3H -TdR掺入和流式细胞仪分析法。结果 :NGF(1～ 10 0U/ml)可明显促进培养的人牙乳头间充质细胞的3H -TdR掺入率。NGF(10 0U/ml)刺激细胞增殖作用最强。NGF(10 0 0U/ml)抑制细胞的增殖。在NGF(10 0U/ml)作用下 ,细胞S %明显升高 ,细胞增殖指数 (S +G2 M) %也明显增高。结论 :神经生长因子可促进人牙乳头间充质细胞DNA合成 。
AIM: The effects of nerve growth factor on the proliferation and cell cycle of human dental papilla mesenchyme cells were detected to evaluate its possible roles in tooth development. METHODS: 3H-TdR incorporation and flow cytometer analysis. RESULTS: The nerve growth factor showed a significant concentration-dependant stimulation of [ 3H] thymidine uptake, with a maximum increase at 100U/ml, but inhibit the cellular proliferation at 1000U/ml. Nerve growth factor at 100U/ml increased the percenage of cells in S cycle phase and the percentage of S+G 2M. CONCLUSION: nerve growth factor stimulated DNA synthesis of human dental papilla mesenchyme cells, and stimulated the transformation of cells from G 1 to S cycle phase.
出处
《牙体牙髓牙周病学杂志》
CAS
2001年第1期38-40,共3页
Chinese Journal of Conservative Dentistry
