摘要
目的 研究血管内皮生长因子 (VEGF)反义寡核苷酸 (ODN)是否可以抑制人肝癌细胞系VEGF的蛋白表达 ,进而抑制肝癌的血管形成 ,探讨肝癌基因治疗的新方法。方法 人肝癌细胞系 772 1能表达VEGF ,其培养上清液对牛主动脉内皮细胞 (BAEC)的生长有促进作用。采用人工合成反义、正义ODN分别加入 772 1细胞培养液 ,比较 772 1细胞VEGF的表达以及各上清液刺激内皮细胞生长的受抑情况 ,即可了解ODN通过抑制VEGF的表达抑制肝癌的血管形成情况。结果 VEGF反义ODN明显抑制了人肝癌细胞VEGF的表达 (灰度值 :反义组 12 2 6 ,正义组 10 1 3 ,对照组 10 6 3,P <0 0 1) ,且抑制率与剂量呈线性关系 (当剂量为 1、2 5、5、10 μmol/L时抑制率分别为 :6 3 2 %、82 4%、2 10 0 %、2 87 5 % )。结论 VEGF反义ODN能够抑制肝癌的血管形成 。
Objective To investigate whether or not the antisense oligodeoxynucleotide (ODN) of vascular endothelial growth factor (VEGF) can supresses the protein expression of VEGFMethods SMMC 7721 is a cell line that expresses VEGF. Therefore the medium conditioned by SMMC 7721 stimulates proliferation of bovine aortic endothelial cell (BAEC). Synthesized antisense ODN complementary to one region of VEGF mRNA was added into the medium of SMMC 7721. The expression of VEGF and the stimulation effect of SMMC 7721 was then determined Result It was found that by means of inhibition of VEGF expression, the stimulation effect of SMMC 7721 was inhibited by the VEGF antisense ODN in a dose dependent manner. At a dosage of 1, 2 5, 5, 10?μmol/L, the inhibition rate was 63 2%, 82 4%, 210% and 287 5%, respectively. Conclusion It is promising that VEGF antisense ODN works as a new gene therapeutic modaliey for antiangiogenesis of HCC.
出处
《中华普通外科杂志》
CSCD
北大核心
2001年第4期222-224,共3页
Chinese Journal of General Surgery
关键词
反义寡核苷酸类
内皮生长因子
血管形成
免疫抑制因子
肝细胞癌
新生血管化
Oligonucleotides, antisense
Endothelial growth factors
Suppressor factors, immunologic
Carcinoma, hepatocellular
Neovascularization
