摘要
目的 了解高眼压状态下视网膜 N-甲基 - D-天门冬氨酸受体 (N- methyl- D- aspartatereceptor,NMDAR)的功能亚单位 NMDAR1基因 m RNA表达的情况。 方法 采用逆转录多聚酶链反应(reverse transcription polymerase chain reaction,RT- PCR)、β-肌动蛋白 (β- actin)作内对照对高眼压模型眼视网膜内 NMDAR1基因的 m RNA进行半定量分析。16只大白兔的 2 6只眼分成 3组。实验组 10只兔 10只眼 ,前房穿刺生理盐水高压 6 0 mm Hg(1mm Hg=0 .133k Pa)灌注 ,持续 4h。阳性对照组 10只兔 10只眼 ,为实验组的对侧眼 ,用实验组相同的方法以 2 0 mm Hg的压力前房灌注持续 4h作阳性对照。正常对照组 6只兔 6只眼 ,不作眼球灌注处理。 结果 实验组的高眼压灌注眼与阳性对照眼 NMDAR1的 m RNA的水平无明显差异 (P>0 .0 5 )。 结论 急性高眼压未改变兔视网膜 NMDAR1基因的 m RNA表达水平。
Objective To quantify the mRNA expression of NMDAR1 gene in the retina of eyes with acute elevation of IOP in rabbit. Methods Tweenty-six eyes of 16 rabbits were divided into three groups: Group 1: The IOP of one eye in 10 rabbits was elevated to 60 mm Hg by anterior chamber infusion. Group 2: The another eye of the same rabbit in group 1 was maintained the IOP to 20 mm Hg by anterior chamber infusion. Group 3: Unilateral eyes of six rabbits were enucleated to evaluate the mRNA levels as normal control group. PCR product was identified by Southern blotting and the mRNA expression level was quantified by RT-PCR. Results The results revealed no significant difference between group 1 and group 2. Conclusion This implies that acute elevated IOP may not affect the mRNA expression level of NMDAR1 gene.
出处
《中华眼底病杂志》
CAS
CSCD
2001年第1期50-51,共2页
Chinese Journal of Ocular Fundus Diseases
