摘要
利用套叠 PCR技术 (又称重叠区扩增基因拼接法 )对 h GM-CSF基因内第 2 8位氨基酸处的糖基化位点进行突变和进行人促性腺激素基因、腺苷酸激酶短肽与胰岛素样生长因子 -I基因三者之间的拼接 ,结果表明采用该技术能在体外实行有效的基因重组和定点突变 ,其成功率为 1 0 0 % ;这一技术不需要内切酶消化和连接酶处理 ,技术操作简单易行 ,在基因拼接。
The glycosylation site (at the 28th amino acid residue) of human granulocyte macrophage colony stimulating factor (hGM CSF) gene was mutated by gene SOEing.Follicle stimulating hormone gene (FSH),short peptide of adenine nucleotide kinase and insulin like gowth factor I gene (IGF I) were recombined together by gene splicing by overlap extension (gene SOEing). Results showed that gene SOEing was a successful specific PCR technique for gene recombination and gene mutation in vitro . The technique didnt need restrict enzymes and ligases,which could be developed as a simple and useful technique in the studies of gene splicing and gene mutation.
出处
《生命科学研究》
CAS
CSCD
2001年第1期52-55,共4页
Life Science Research
基金
海南省自然科学基金资助项目
海南医学院科学基金资助项目
