摘要
采用逆转录 -聚合酶链式反应 (RT PCR)方法 ,从草鱼肝脏的总RNA中扩增出胰岛素样生长因子 I(IGF I)基因序列 ,定向克隆至质粒pUC18。测定了该基因序列 ,推导其编码的蛋白质序列。克隆的cDNA序列编码包括B、C、A、D和E五个区域的 117个氨基酸。与鲤IGF I成熟肽比较 ,核酸序列和氨基酸序列的同源性分别为 93.8%和 97.1%。E区域分析结果表明 ,所克隆的草鱼IGF I序列属于IGF IEa
Total RNA was isolated from grass carp (Ctenopharyngodon idellus) liver tissue. The cDNA encoding insulin like growth factor I (IGF I) peptide was amplified by reverse transcription polymerase chain reaction (RT PCR) strategy using isolated total RNA as template. The amplified cDNA fragment was inserted to vector pUC18. The cloned cDNA was sequenced and the amino acid sequence of grass carp IGF I was predicted. The nucleotide sequencing showed the cDNA encoded 117 amino acids including B,C,A,D and E five domains. The nucleotide sequence and deduced amino acid sequence shared 93.8% and 97.1% homology with that of common carp mature IGF I. Analysis of E domain indicated that cloned grass carp IGF I belonged to IGF IEa 2 subtype.
出处
《水产学报》
CAS
CSCD
北大核心
2001年第1期1-4,共4页
Journal of Fisheries of China
基金
农业部"九五"重点渔业科技资助项目! (渔 95 -B -96 -0 2 -0 8-0 2 )
