摘要
通过T3菌苗免疫中华鳖后的血清间接凝集抗体效价 (IAT)与免疫保护率 (PRP)的变化探讨了中华鳖的免疫应答规律。中华鳖对T3菌苗能产生较强的应答反应 ,免疫的二龄鳖PRP可达 82 6± 15 2 (19) % ,IAT达 1978 1± 716 4(19)。它对T3菌苗的免疫效应期为 3天 ,第 2 0天时免疫应答处于高峰 ,IAT与PRP分别为 176 8 0± 44 7 6 (4)与 91 7± 14 4(4) % ,持续 10天左右开始下降 ,到第 3个月左右基本上回复到免疫开始时水平。结论为 :中华鳖的免疫应答反应基本介于鱼类与鸟类之间 ,但较偏向于鸟类。
The immune response in soft\|shelled turtle (Trionyx sinensis) immunized with bacterin T3, was determined with indirect agglutination titer (IAT) and percent relative protection (PRP). In the vaccinated turtle, the strong immune response to bacterin T3 was produced. Six groups of 1\|year\|old turtles and 19 groups of 2\|years\|old turtles were respectively vaccinated by bacterin T3. At the 30 th day, the PRP and IAT of the 1\|year\|old turtles were to 61 8±11 2(6)% and 403 3±165 4(6), yet the corresponding values of 2\|years\|old turtles were 82 6±15 2(19)% and 1?978 1±716 4(19) respectively. According to the test, the PRP and IAT of 2\|years\|old turtles were higher than those of 1\|year\|old turtles. The study demonstrated that the effective phase of immune response to bacterin T3 was 3 days. At the 20 th day, the IAT value is up to 1?768 0±447 6(4). Which maintained for about 10 days and decreased to the initial level on the 60 th day. The results proved that the immunologic memory of the turtles was strong. The secondary enhanced immunization began at the 30 th day of the primary immunization. The IAT increased quickly and was 5?760 0±2?790 0(4) at the 16 th day of the secondary immunization, which reached the second peak value that was nine times as high as the primary peak value. The IAT decreased to 4?266 7±1?478 0(3) at the 30th day, 2?986 5± 1?955 3 (3) at the 60 th day, and 1?280 0±0(2) at the 70 th day. The result still has no significant difference compared to the peak value of the secondary immunization (0 2< P <0 3), yet having significantly higher than that of immunization (0 01< P <0 02). From these facts, we can concluded that the decreasing speed of antibody of the secondary immunization was clearly slower than increasing speed. The result suggests that the character of immune response of Soft\|shelled turtle lies between fish and bird, but it is more like the latter.
出处
《动物学报》
SCIE
CAS
CSCD
北大核心
2001年第2期163-169,共7页
ACTA ZOOLOGICA SINICA
基金
国家自然科学基金资助项目!(No .3 95 70 5 65 )
农业部"九五"重点项目!(渔 95 A 96 0 3 0 4)资助&&
关键词
中华鳖
T3菌苗
免疫应答
Soft\|shelled turtle (Trionyx sinensis), Bacterin T3, Immune response
