摘要
研究了４株可用作饲料添加剂的纤维素分解菌，在不同发酵时间、发酵温度和厌氧发酵条件下，菌体蛋白含量、纤维素酶和半纤维素酶活性等的变化。结果表明：发酵２０ｈ酶活性最高；供试菌都适宜在２８℃恒温发酵；在厌氧条件下，３９℃±℃发酵１２ｈ、２４ｈ、３６ｈ，供试菌均可在ＰＤＡ平板上正常生长，发酵４８ｈ供试菌生长和酶活均受不同程度的影响。研究为菌株的生产、保存和应用提供了重要依据。
Four cellulolytic strains, which can be used as feed additive, were studied under the condi-- tions of various temperature, incubation time, and anaerobic process, and examined the changes of their cell protein content, cellulase and hemi-cellulase activity. The results show: 1) The maximum cellulolytic enzyme activities were observed incubation 20h; 2) Constant medium temperature 28℃ was adequate to the growth of the 4 strains; 3) anaerobic condition, 39℃±2℃ and fermentation 12h, 24h, 36h, the tested strains can growth well in PDA plate, however, the cellulolytic enzyme activities and growth of the tested strains were influenced adversely when fermentation 48h. The experiment provide many important basis for the strains production, storage and utilization.
出处
《微生物学通报》
CAS
CSCD
北大核心
2001年第2期30-33,共4页
Microbiology China
基金
山西省自然科学基金资助项目!(No.971079)
