摘要
应用补体介导细胞毒法体外净化骨髓中残留白血病细胞后,对普通型急性淋巴细胞白血病(CALL)患者4例进行了自身骨髓移植(ABMT)。单个核细胞经单克隆抗体55(McAb 55)加兔补体两次处理后,CALL抗原阳性细胞清除率可达3个对数级,骨髓有核细胞及CFU-GM回收率分别为25.8％～64.8％和40.0％～62.4％,外周血白细胞于移植后第11～13天开始回升,15～29天升至10~9/L以上。第23～53天出洁净室,现无病存活时间分别为8^+、7^+,3^+和1.5个月,远期疗效仍在定期随访中。
A monoclonal antiooay (McAD 00.1 specinc lor tne common acute-lympnoDlastic-leuKaemra antigen (CALLA) and rabbit complement were used for in vitro treatment of bone marrow before autologous transplantation in four patients suffering from CALLA-positive acute lymphoblastic leukaemia in rescission.The bone marrow treated was preserved at room temperature (21-24℃) for 56 hours.The patients were treated with cyclophosphamide [40-50mg/(kg.d) for 2 days] and total body irradiation (600-700) cGy in one day first),and then with transplantation of autologous bone marrow treated with the McAb and complement.The transplantation was well tolerated and full bone marrow recon-stitution was observed in all the four patients.They are surviving and leukaemiafree at 8,7,3and 1.5 months posttransplantation.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
1989年第6期434-437,共4页
Medical Journal of Chinese People's Liberation Army
关键词
白血病
治疗
骨髓体外净化
自身骨髓移植
Acute lymphoblastic leukaemia In vitro treatment of bone marrow Bone marrow autotransplantation
