摘要
目的 为了解腔阔盘吸虫在贝类宿主体内发育的组织学情况。方法 用腔阔盘吸虫 (Eurytremacoelomaticum)虫卵人工感染同型阔纹锅牛BradybaenasimilarisFerussac后在不同时间里固定蜗牛 ,经石蜡包埋 ,连续切片、苏木精伊红复染 ,观察腔阔盘吸虫在蜗牛体内的发育变化。结果与结论 感染后 5～ 12天为早期母胞蚴阶段 ,感染 15天母胞蚴体内开始出现子胞蚴的胚球 ,2 9天的母胞蚴增大明显 ,胚球数目增多 ;40天的母胞蚴布满肠壁周围 ,胚球两端拉长 ;47天的母胞蚴体内的子胞蚴胚球增大明显 ,已出现内外囊壁的分化 ,内囊壁包围着胚细胞组成的中心区域 ;感染 91天的子胞蚴体内出现尾蚴胚体 ;118天的子胞蚴体内的尾蚴已经成熟 。
Aim This paper reported the histological development of Eurytrema coelomaticum in the snails Bradybaena similaris Ferussac.Method Snails were infected experimentally by feeding Eurytrema coelomaticum eggs,5～6 snails were fixed in Bouin's fluid at intervals of 3～6 days postinfection,the entire animal was dehydrated in thanol-toluol series,embedded in paraffin,serially sectioned at 10μm,and stained with Enlirch's hematoxylin-eosin.Results and Conclusion the germinal cells in the mother sporocyst stretched and scattered in 5～12days postinfection snails,and the number of germninal cells increased.In the 15 days postinfection snails,germinal cells created a large space around themselves and germinal balls were presented in mother sporocyst,each become a daughter sporocyst.At 29 days postinfection mother sporocyst enlarged obviously,the mumber of germinal balls increased.At 40 days postinfection,mother sporocyst scattered all over the intestine wall.The germinal balls lengthened at the both sides.By 47 days postinfection,the germinal balls in mother sporocyst enlarged significantly,daughter sporocyst balls present internal and external cyst walls,the internal cyst wall surrounded the central area that the germ cells aggregate.After 3 months postinfection,daughter sporocyst present the cercarial embroys.At 118 days postinfection,the cercariae in daughter sporocyst were mature and daughter sporocyst moved to pulmonary cavity.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2001年第2期46-49,共4页
Chinese Journal of Zoonoses
关键词
腔阔盘吸虫
幼虫期
同型巴蜗牛
组织学
贝类宿主
Eurytrema coelomatium
Larval stage
Bradybaena similaris Ferussac
Histology
