摘要
目的 探讨HIV/AIDS患者T淋巴细胞亚群、表型和CD4 凋亡细胞之间的相关性及其临床意义。方法 用流式细胞仪法对HIV/AIDS患者和年龄相配的HIV阴性人群的T淋巴细胞亚群、表型和CD4 凋亡细胞的绝对和相对计数进行测定 ,比较这些指标在两组人群中的差别和分析各项指标之间的相关性。结果 在HIV/AIDS患者组 ,CD2 5细胞表型与CD4 计数呈正相关 ,HLA DR细胞表型的绝对计数也与CD4 计数呈正相关 ,但其百分率却与后者无相关性。与对照组比较 ,患者组的CD4 和CD2 5双阳细胞 (CD+ 4、CD+ 2 5)百分率降低而总体HLA DR水平升高。在患者组CD4 细胞凋亡百分率与CD4 计数呈负相关。结论 与CD4 计数和百分率以及CD4 和CD8的比值相结合 ,CD2 5水平有助于判断HIV感染的进程和判断疗效。CD4细胞凋亡可能有助于判断晚期艾滋病。
Aim To determine correlation of absolute counts and percentages of CD_4 and CD_8 lymphocyte subsets and their phenotypes (CD_ 25 and/or HLA-DR-expressing cells) as well as apoptotic CD_4 cell so as to look for which of them can be used separately or combining with others to predict and judge progression of HIVinfection and effectiveness of treatment for the disease.Methods Flow cytometry was used to measure levels of absolute counts and percentages of CD_4 cell,CD_8 cell and CD_ 25 expressing cells,HLA-DR-expressing cells in total lynnphocytes,in CD_4 cells, in CD_8 cells and in other kinds of lymphocyte subsets as well as apoptotic CD_4 cells in HIV/AIDS patients compared with age-matched HIV-negative subjects. Results Levels of both absolute counts and percentages of CD_ 25 expressing cells in total lymphocytes,in CD_4 cells and in some other lymphoid subsets positively correlated to level of CD_4 count,levels of absolute counts of HLA-DR-expressing cells in total lymphocytes,in CD_8 cells and in some other kinds of cells also positively correlated(but their percentages did notcorrelate) to CD_4 count. The percentage of CD_4 and CD_ 25 double positive(CD+_4、CD+_ 25)cells decreased but that of total HLA-DR increased in HIV/AIDS patients compated with HIV-negative subjects. Level of absolute count of apoptotic CD_4 cell positively but its percentage negatively correlated with level of CD_4 count although both forners were higher in patient group than in control group.Conclusions It seems that level of CD_ 25 may be used to predict progression of HIV infection when combining with levels of count and percentage of CD_4 cells as well as ratio of CD_4 to CD_8;it may be a good thing to HIV/AIDSpatients if level of CD_ 25 increase after a treatment.Apparent increase of apoptotic CD_4 cell percentage may help judging full-blown AIDS when combining with other parameters.
出处
《中国人兽共患病杂志》
CSCD
北大核心
2001年第2期3-7,共5页
Chinese Journal of Zoonoses
