摘要
目的研究肺结核患者外周血CD4+T淋巴细胞凋亡与结核病发病机制的关系。方法分离并标记肺结核患者和健康人外周血单个核细胞,采用流式细胞仪测定CD4+T淋巴细胞凋亡率。结果肺结核患者及其涂阳组、涂阴组、初治组、复治组外周血CD4+T淋巴细胞凋亡率分别为(15.882±4.650)%、(15.089±4.179)%、(16.137±5.656)%、(15.129±3.7000)%和(17.819±6.678)%,与对照组CD4+T淋巴细胞凋亡率(8.330±4.196)%比较差异均有统计学意义(P<0.01),涂阳组与涂阴组比较差异无统计学意义(P>0.05),初治组与复治组比较差异无统计学意义(P>0.05)。结论肺结核患者外周血CD4+T淋巴细胞凋亡率显著增加,CD4+T淋巴细胞数量减少,但CD4+T淋巴细胞凋亡率与患者是否处于疾病的活动期及治疗情况关系不大。
Objective To study the apoptosis of CD4＋ T lymphocytes in patients with pulmonary tuberculosis and ex- plore its relationship to the pathogenesis of tuberculosis. Methods Mononuclear cells in blood from patients with tuber- culosis and healthy individuals were isolated and labeled. Flow cytometry was used to measure the percentage of apoptotic CD4＋T lymphocytes. Results The rate of CD4＋T lymphocyte apoptosis was （15. 882±4. 650）%0 in patients with tu- berculosis（ 15. 089±4. 179） % in the smearlpositive group, （16. 137±5. 656） % in the smear-negative group, （15. 129 ±3; 7000）% in the initial treatment group, and（17. 819± 6. 678）% in the repeat treatment group. These rates differed significa＇ntly （P〈0.01） compared to rate of CD4 ＋T lymphocyte apoptosis in the control group of （8. 330±4. 196）%. Rates for the smear-positive group and smear-negative group did not differ significantly （P〉0.05）, and rates for the ini- tim treatment group and the repear treatment group did not differ significantly （P〉0.05）. Conclusion The rate of CD4＋ T lymphocyte apoptosis in patients with tuberculosis was significantly higher than in healthy individuals and the number of CD4＋ T lymphoeytes decreased, but the rate of CD4＋ T lymphocyte apoptosis has little relationship to the ac- tive phase of tuberculosis and the state of treatment.
出处
《中国病原生物学杂志》
CSCD
北大核心
2013年第4期322-324,共3页
Journal of Pathogen Biology
基金
济宁市科技局资助项目(No.2010018)
