摘要
目的 探讨急性早幼粒细胞白血病 (APL)患者用全反维甲酸 (ATRA)和砷剂 (AS2 O3)治疗期间细胞组织因子 (TF)表达及血浆止凝血分子标志物含量变化。方法 用ELISA法检测血浆TF、组织因子途径抑制物 (TFPI)、凝血酶 抗凝血酶复合物 (TAT)、纤溶酶 抗纤溶酶复合物 (PAP)、尿激酶型纤溶酶原激活物 (u PA)、尿激酶型纤溶酶原激活物受体 (u PAR)和细胞裂解液TF含量 ;RT PCR法检测细胞TFmRNA转录水平。结果 治疗前 ,患者血浆TF[(98 3± 19 8)ng/L、(89 6± 15 2 )ng/L]、TFPI[(94 4± 37 0 )mg/L、(93 5± 36 4)mg/L]、TAT [(2 1 9± 9 6 ) μg/L、(18 2± 9 7) μg/L]、PAP[(0 73± 0 2 6 )mg/L、(0 6 3± 0 33)mg/L]、u PA[(0 6 3± 0 2 3) μg/L、(0 5 7± 0 0 1) μg/L]、u PAR[(0 41± 0 14) μg/L、(0 47± 0 16 ) μg/L]水平与正常对照组相比为高 ,差异有显著性或极显著意义(P <0 0 5～ 0 0 1)。骨髓分离的单个核细胞TFmRNA转录、裂解液TF水平 [(6 80 2 4± 45 6 6 1)pg/10 7、(36 8 0 2± 15 1 2 )pg/10 7]与对照组相比均显著升高 ;经ATRA、AS2 O3 治疗后显著下降。结论 APL患者TF表达增高、凝血系统激活、纤溶亢进 ,但随病情好转可逐渐改善。ATRA及AS2 O3
Objectives To study the changes of tissue factor expression and hemostatic molecular markers in acute promyelocytic leukemia during all trans retinoic acid(ATRA)or arsenic trioxide(AS 2O 3) treatment Methods The plasma level of tissue factor (TF)、tissue factor pathway inhibitor(TFPI)、thrombin antithrombin complex(TAT)、plasim antiplasim complex(PAP)、 urokinase type plas minogen activator(u PA)、urokinase type plas minogen activator receptor(u PAR) and the TF level of bone marrow blasts lysate were measured by ELISA Ttranscription of TF mRNA was detected by RT PCR Results The plasma levels of TF [(98 3±19 8) ng/L、(89 6±15 2) ng/L]、TFPI[(94 4±37 0) mg /L、(93 5±36 4) mg/L]、TAT[(21 9±9 6) μg/L、(18 2±9 7) μg /L、 PAP[(0 73±0 26) mg/L、(0 63±0 33) mg /L]、u PA[(0 63±0 23) μg/L、 (0 57±0 01) μg/L] and u PAR[(0 41±0 14) μg/L、(0 47±0 16) μg/L],the TF of bone marrow blasts lysate[(680 24±456 61) pg/10 7、(368 02±151 2) pg/10 7] and transcription of mRNA were all remarkably evelated at the time of diagnosis They all decreased after ATRA and AS 2O 3 administration. Conclusions There is over expression of TF、activation of coagulation system and hyperfibrinolysis, in patients with acute promyelocytic leukemia,these can be ameliorated with clinical improvement All trans retinoic acid and arsenic trioxide down regulate the expression of TF mRNA and decrease the TF contents in APL blasts However, there is also high plasma level of TF and TAT indicating the existence of hypercoagulability after remission
出处
《中华内科杂志》
CAS
CSCD
北大核心
2001年第1期36-39,共4页
Chinese Journal of Internal Medicine
