摘要
目的 :研究了OKT3所致的体内T细胞抗原调变作用。方法 :采用流式细胞术免疫荧光三色分析法 ,动态监测了19例肾移植术后应用OKT3治疗的患者的T细胞及其亚群的表达率和CD3抗原荧光强度的变化。结果 :当注射OKT3后 ,CD3+ 、CD3+ CD4+ 、CD3+ CD8+ 细胞百分率明显下降甚至消失 ,相对地出现CD3- CD4+ 和CD3- CD8+ 细胞 ;同时 ,CD3+ 细胞的荧光强度也较注射前下降了 86 %;CD3+ CD4+ CD3+ CD8+ 比值以及CD3- CD4+ CD3- CD8+ 比值波动均很大 ,甚至表现为明显的倒置。当停用OKT3后 ,CD3+ 、CD3+ CD4+ 和CD3+ CD8+ 细胞逐渐回升 ,而CD3- CD4+ 和CD3- CD8+ 细胞开始下降直至消失 ;同时CD3+ 细胞的荧光强度逐渐恢复正常 ;CD3+ CD4+ CD3+ CD8+ 比值也很快恢复正常 ,但CD3- CD4+ 和CD3- CD8+ 比值倒置更明显 ,直至为 0。结论 :OKT3所致的T细胞抗原调变会出现CD3- CD4+ 和CD3- CD8+ 细胞 ,提示使用CD4
Objective:To study in vivo antigenic modulation of human T lymphocytes induced by OKT3.Methods:We monitored T lymphocytes and subsets of 19 OKT3 treated kindey graft recipients by three color flow cytometry.T cell counts and fluorescent intensity were done on the day before the first OKT3 injection,one day,three days and five days during OKT3 injection, one day and five days after OKT3 injection.Results:The percentage of CD3 +,CD3 +CD4 + and CD3 +CD8 + cells were dramatically decreased and disappeared following the first injection of OKT3, and the fluorescent intensity of the CD3 + cells were also significantly decreased 86%,while modulating CD3 -CD4 + and CD3 -CD8 + cells were observed but were functionally inactive. After OKT3 was stopped, CD3 +,CD3 +CD4 + and CD3 +CD8 + cells were gradually increased, the fluorescent intensity of the CD3 + cells began to evaluate, while CD3 -CD4 + and CD3 -CD8 + cells were decreased and vanished.Conclusion:Antigenic modulation of human T lymphocytes by OKT3 results in an appearance of modulating cells,it suggests that accurately monitoring CD4/CD8 should be done in transplant rejection.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2001年第3期161-163,共3页
Chinese Journal of Immunology
