摘要
目的 观察TNP 470对人恶性胶质瘤细胞系SHG 44体内和体外生长的影响。方法 采用MTT法、软琼脂克隆法、流式细胞仪、光镜和透射电镜技术观察SHG 44细胞体外增殖、克隆形成、细胞周期和形态学变化及异种移植瘤生长情况。结果 2 0～ 2 0 0 0ng/mlTNP 470可显著抑制SHG 44细胞体外增殖 ,克隆形成率显著降低 ,G0 /G1期细胞明显增多 ,S、G2 /M期细胞减少。采用30mg/kg体重TNP 470隔日皮下注射后 ,移植瘤体积缩小、重量显著减轻 ,组织出现明显凋亡细胞和坏死。结论 TNP 470对SHG 44细胞的生长抑制作用与其调控细胞周期和诱导细胞凋亡相关 ,提示TNP
Objective To investigate the effects of TNP 470 on the growth of a human malignant glioma cell line SHG 44 in vivo and in vitro . Methods The colorimetric MTT assay, soft agar culture, flow cytometry,light and electron microscopy were used to determine the proliferation, the cloning efficiency, cell cycle and the morphological changes of SHG 44 cells as well as the growth of its xenografted tumor. Results TNP 470 (20～2 000 ng/ml) significantly inhibited the proliferation of SHG 44 cells in vitro (the 50% inhibitory concentration was 200 ng/ml). Cloning efficiency reduced obviously. The number of cells in G 0/G 1 phase increased, while that in S, G 2/M phases decreased significantly. Weight and volume of xenografted tumors treated with TNP 470 (30 mg/kg, injected subcutaneously every other day) reduced notably. Furthermore, there were necrotic area and apoptosis in the tumor. No severe side effect of TNP 470 was found in this study. Conclusion The inhibitory effect of TNP 470 on the growth of SHG 44 cells correlates with its functions of regulating cell cycle and inducing apoptosis, which suggests that the angiogenesis inhibitor TNP 470 has strong inhibitory effect on human malignant gliomas.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2001年第3期272-275,共4页
Journal of Third Military Medical University
基金
国家自然科学基金资助项目 !(3 9570 2 95)
