摘要
目的研究系统性红斑狼疮（ SLE）患者 T细胞的克隆性。方法用逆转录－聚合酶链反应和基因扫描技术分析了 6例活动性 SLE患者外周血淋巴细胞的 T细胞克隆性。结果 2例 SLE患者的 Vβ产物来自多克隆 T细胞，另 4例患者的 Vβ 9、 Vβ 2、 Vβ 1、 Vβ 2产物均来自寡克隆 T细胞。结论 SLE患者 T细胞存在着克隆性增殖，并有可能是特定的自身抗原所引起的一种特异性应答。
Objective To investigate the T cell clonality in patients with systemic lupus erythematosus (SLE). Methods T cell clonality in peripheral blood lymphocytes from six patients with active SLE was analyzed using reverse transcriptase-polymerase chain reaction (RT-PCR) and GeneScan technique. Results T cell receptor (TCR) Vβ PCR products from two patients were derived from polyclonal T cells, however, TCR Vβ 9, Vβ 2, Vβ 1, Vβ 2 products from the other four patients were derived from oligoclonal T cells respectively. Conclusion T cells from patients with SLE demonstrated a striking oligoclnality and their clonal expansion may be a specific response driven by autoantigens.
出处
《中华皮肤科杂志》
CAS
CSCD
北大核心
2001年第1期15-17,共3页
Chinese Journal of Dermatology
基金
中华医学会皮肤科学会基金
