摘要
目的 探讨几丁质室修复颞骨内面神经缺损的可行性。方法 采用生物材料几丁质室桥接兔骨内面神经缺损 2 4只 (侧 ) ,硅胶室作为对照组 2 4只 (侧 ) ,术后 1、3和 5个月对再生神经分别进行大体观察、电生理测试、组织学检查以及数字形态学分析。结果 ①术后 3个月 ,近端神经纤维已通过几丁质室进入远端神经 ,术后 5个月 ,新生神经更显成熟 ;②神经诱发电位显示再生神经传导功能恢复良好 ,实验组和对照组之间差异无显著性 ;③再生神经均以有髓神经纤维为主 ,轴突发育良好 ,超微结构显示髓鞘板层明暗相间、结构清晰 ,轴浆细胞器丰富 ;④术后 5个月 ,几丁质组再生神经纤维面积恢复率、轴突面积恢复率及轴突数目恢复率均达 71%以上 ,与硅胶组比较差异均无显著性。结论 几丁质室修复颞骨内面神经缺损可取得良好的再生效果。
Objective To investigate the feasibility of intratemporal facial nerve regeneration in a chitin chamber. Methods A left 6 mm intratemporal facial nerve gap was created in adult white rabbit, and a total of 48 animals were used. The proximal and distal stumps were bridged with chitin chamber (experimental group) and silicone chamber (control group). Regeneration of the nerve was assessed by gross observation, nerve electrophysiological test, histological examination and digital morphological analysis in both groups. Results ① Three months after implantation, the proximal transected neural stump was connected with the distal stump in the chitin chamber. Five months after operation, the regenerated nerve exhibited a more mature fascicular organization and the chitin tubes were completely absorbed. ② Nerve evoked potentials showed better conduction ability in the chitin chamber. There were no significant difference in electrophysiological indices between the two chamber groups. ③ Most of the regenerated nerve fibers were myelinated nerve fibers, and axons and myelins had similar histograms between the experimental and control groups. ④ Five months after operation, the mean percentage of recovery of the regenerated nerve fiber area, axonal area and axonal number were more than 71% in the experimental group. There was no significant difference between the control and experimental groups. Conclusion These results suggested that intratemporal facial nerve could regenerate effectively within the chitin chamber in rabbits and such kind of chamber may be clinically useful.
出处
《中华耳鼻咽喉科杂志》
CSCD
北大核心
2001年第2期97-100,T003,共5页
Chinese Journal of Otorhinolaryngology
基金
湖北省教育委员会青年基金!资助课题 (未设编号 )
