摘要
目的 研究肺腺癌细胞的核形态及其 bcl- 2和 nm2 3蛋白的表达情况 ,探讨其与肺腺癌生物学行为及预后的关系。方法 采用免疫组化 S- P法 ,对 41例肺腺癌及其癌旁正常肺组织中 bcl- 2、nm2 3蛋白进行检测 ,并用计算机图像分析系统对其细胞核进行测量。结果 肺腺癌细胞核形态参数值 (面积、周长、等效直径、圆形度及异型指数 )明显高于正常肺上皮细胞 (P<0 .0 1) ,而与肺腺癌分化程度、转移及预后无相关性 (P>0 .0 5 ) ;bcl- 2表达与预后呈负相关 (P<0 .0 5 ) ,与分化、转移无关 ;nm2 3与淋巴结转移及预后呈正相关 (P<0 .0 5 )。细胞核形态参数与nm2 3表达无关 ,其中的核面积、周长及等效直径与 bcl- 2呈正相关 (P<0 .0 5 )。结论 细胞图像分析可作为鉴别肿瘤良、恶性的一种手段。癌细胞核越大 ,凋亡受抑 ;bcl- 2及 nm2 3蛋白的存在在肺腺癌的发展过程中起了重要作用。
Objective To observe the expression of bcl 2,nm23 protein and the morphology of cell nucleus in human lung adenocarcinoma(LAC),study their significance in biological behavior and prognosis of LAC.Methods Forty one cases of LAC tissue and tumor surrounding normal tissue were examined with immunohistochemistry,and the morphology of cell nucleus was observed by cell imagine analyses.Results The morphology of cell nucleus including area,circumference,cycle degree and atypical degree was not related to differential degree,metastasis and prognosis of tumors.The expression of bcl 2 protein was related to prognosis and the expression of nm23 was related to metastaeis and prognosis.The morphology of adenocarcinoma cell nucleus was not related to the expression of nm23.Conclusion The analysis of cell imagine can be used to distinguish the benign tumors from the malignant.The expression of bcl 2 and nm23 protein is correlated with the development of LAC,and has important value in prognosis of patients with LAC.\;
出处
《实用肿瘤杂志》
CAS
北大核心
2001年第2期93-96,共4页
Journal of Practical Oncology
