摘要
目的 研究催乳素对Graves病 (GD)甲状腺细胞与自体外周血单个核细胞 (PBMC)在体外共同培养时相互作用的影响。方法 利用免疫荧光染色和流式细胞仪等 ,测定在不同羊催乳素(oPRL)水平进行共同培养时PBMC的活化、增殖反应和甲状腺细胞对人类白细胞抗原基因复合体(HLA) DR及CD4 0 的表达。结果 oPRL浓度为 2 0 0 μg/L时与GD甲状腺细胞共同培养的PBMC中CD4 +CD2 5+细胞百分率 [(13 0 8± 2 5 4) % ,P <0 0 1]和增殖指数 [(17 82± 3 0 2 ) % ,P <0 0 1]及 10 0 0μg/L时的增殖指数 [(16 5 7± 2 5 6 ) % ,P <0 0 5 ]均较 0 [分别 (10 15± 2 6 0 ) %和 (14 38± 2 6 4) % ]、12 5及 5 0 μg/L时有显著增加。在 2 0 0和 10 0 0 μg/L时相应甲状腺细胞中CD4 0 +细胞百分率 [(4 8 2 5± 6 6 3) % ,(5 2 2 8± 6 94) % ]和平均荧光强度 (dMF ;42 94± 10 2 4,49 5 1± 12 34)明显低于 0 [(5 8 38± 6 6 2 ) %和 6 7 30± 2 0 2 0 ]、12 5及 5 0 μg/L剂量组。而在 5 0 μg/L时其HLA DR+细胞百分率[(4 6 79± 7 5 1) % ,P <0 0 1]和dMF(2 1 0 2± 5 43 ,P <0 0 1)显著高于 0 [(33 5 1± 8 5 8) %和 13 91±3 88]、12 5、2 0 0及 10 0 0 μg/L剂量组。除此以外 。
Objective To study the effects of prolactin on interactions between Graves′ disease (GD) thyrocytes and autologous peripheral blood mononuclear cells (PBMC) in coculture Methods Primary thyrocytes and autologous PBMC were cocultured at various levels of ovine prolactin (oPRL) in vitro The activation and proliferation of PBMC as well as HLA DR and CD 40 expressions on thyrocytes were determined by immunofluorescent staining and flow cytometry Results With PBMC in GD cocultures, oPRL caused significant increase in the percentage of CD 4 +CD 25 + cells at 200 μg/L [(13 08±2 54)%, P <0 01] and in the proliferative index at 200 μg/L [(17 82±3 02)%, P <0 01] and 1 000 μg/L [(16 57±2 56)%, P <0 05], but not at 12 5 μg/L [(10 43±2 38)% and (14 08±2 67)%, respectively] or 50 μg/L [(9 94±2 54)% and (14 56±2 80)%, respectively)] The values at 200 or 1 000 μg/L as above were also significantly different from those at 12 5 or 50 μg/L The percentage of CD 40 + cells [(48 25± 6 63)%,(52 28±6 94)%] and delta mean fluorescence intensity (dMF; 42 94±10 24,49 51±12 34) were significantly lower in thyrocytes in GD cocultures treated with 200 or 1 000 μg/L oPRL than those untreated [(58 38±6 62)% and 67 30±20 20] or treated with two other concentrations of oPRL The percentage of HLA DR + cells [(46 79±7 51)%, P <0 01] and dMF (21 02±5 43, P <0 01) were significantly higher in the thyrocytes treated with 50 μg/L oPRL than those untreated [(33 51±8 58)% and 13 91±3 88] or treated with three other concentrations There were no significant differences between the dose groups of oPRL but those mentioned above Conclusions Prolactin could affect interactions between GD PBMC and autologous thyrocytes and might eventually influence the shift of helper T cells High prolactin levels might aggravate GD
出处
《中华内科杂志》
CAS
CSCD
北大核心
2001年第2期82-85,共4页
Chinese Journal of Internal Medicine
基金
辽宁省自然科学基金资助!(96 2 2 91)
