摘要
目的研究高压氧 (HBO)治疗对 7日龄鼠缺血缺氧性脑损伤后碱性成纤维生长因子 (bFGF)及其 mRNA表达的影响。方法建立新生鼠缺血缺氧性脑病模型，应用 HBO治疗后应用免疫组织化学技术检测脑 bFGF表达，应用原位杂交技术检测脑 bFGF mRNA。结果 HBO组 bFGF表达及 bFGF mRNA表达均显著增强，而高压空气 (HBA)组脑 bFGF表达增强， bFGF mRNA表达减弱。结论 HBO增强新生鼠缺血缺氧性脑损伤后 bFGF及其 mRNA表达。
Objective To study the effect of hyperbaric oxygen on basic fibroblast growth factor(bFGF)and bFGF mRNA expression in 7－ day old neonatal rats after brain hypoxia- ischemia injury.Method Brain hypoxia- ischemia injury in neonatal rats was established by Rice'method After HBO treatment,bFGF expression was investigated by means of immunohistochemical analysis,and bFGF mRNA by means of in situ hybridization.Result bFGF exprssion were higher in both HBO and HBA groups than in the free growing group and controls.However,bFGF mRNA expression increased only in HBO groups.Conclusion HBO increased both bFGF and bFGF mRNA expression of neonatal rats after brain hypoxia- ischemia injury.
出处
《现代康复》
CSCD
2001年第3期48-49,共2页
Modern Rehabilitation
关键词
高压氧
脑缺血缺氧性脑损伤
碱性成纤维生长因子
治疗
hyperbaric oxygen
brain hypoxia- ischemia injury
basic fibroblast growth factor(bFGF)
