摘要
目的 评价Fas和FasL介导细胞凋亡在乙型肝炎肝损伤中的作用及其与HBV抗原的关系。方法 应用免疫组化方法检测了 6 2例乙型肝炎患者肝组织内的Fas、FasL和HBsAg、HBcAg ,并以 6例正常肝组织为对照。结果 Fas/FasL在正常肝组织内无表达 ;Fas主要在乙型肝炎患者肝细胞质内表达 ,5 8例阳性 (93 5 % ) ;FasL在肝内浸润的单个核细胞和肝细胞质内均可见到 ,37例阳性 (5 9 7% )。结论 Fas/FasL的表达程度与肝组织活动性炎症程度密切相关 ;Fas/FasL介导的细胞凋亡可能在乙型肝炎时肝损伤中起重要作用 ;Fas/FasL表达程度与肝内HBV抗原HBsAg。
Objective To evaluate the role of apoptosis mediated by Fas/FasL in the liver of hepatitis B and the expression status of Fas,FasL,and HBV antigens.Methods We studied the expression of Fas antigen,Fas\|ligand (FasL) and hepatitis B virus(HBV) antigens(HBsAg and HBcAg)in the livers of 62 patients with hepatitis B using immunohistochemistry ABC method.Six normal liver samples were used as control.Results Hepatocytes in normal liver had no Fas/FasL expression,but in the cases of hepatitis B,Fas was expressed mainly in the cytoplasm in 58 cases(93\^5%);FasL was observed in infiltrating mononuclear cells and in the cytoplasm of hepatocytes in 37 cases (59\^7%).The expressions of Fas/FasL were closely related to the liver histological inflammation degrees.Conclusion The apoptosis mediated by Fas/FasL may play an important role in the hepatocellular injury in hepatitis B.No correlation was found between the degrees of Fas/FasL expression and the presence of HBsAg and HBcAg in the liver.
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
北大核心
2001年第1期12-15,共4页
Chinese Journal of Experimental and Clinical Virology
