摘要
本文采用了抗性不同的大豆品种 ,在 3～ 4叶期接种 ,并于接种后 1～ 1 0天内连续取样 ,然后测定其接种后叶片内叶绿素的含量。试验结果表明 :无论是抗病品种 ,还是感病品种 ,在病菌侵入的初期 ,叶绿素 a、b的含量都有所下降 ;在病菌侵染后 7～ 1 0天时 ,感病品种叶片内叶绿素 a、b含量比未接种对照低 ,而抗病品种叶片内叶绿素 a、b含量均明显高于未接种对照 ;另外 ,致病力不同的生理小种对叶绿素 a、b含量变化的影响不同 ,7号生理小种对叶绿体的破坏能力最强 ,其次为 6号生理小种 ,而 4号生理小种则最弱 ,其中 1号 ,2号生理小种居中。抗病品种叶片内叶绿素 a/b的比值明显高于感病品种 ,初步认为叶绿素 a/b的比值可作为大豆品种对灰斑病抗性鉴定的一种生化指标。
Soybean varieties of different resistance were inoculated with different races of Cercospora sojina Hara at 3～4 leaf age , were sampled during 1～10 days after inoculation and the content of chlorophyll in leaves of both the inoculated and the healthy plants were measured. The results showed that when the leaves were primarily infected by Cercospora sojina Hara, content of chlorophyll a and b in leaves of resistant varieties and susceptible varieties were lower than those of corresponding healthy leaves. During No.7 day to No.10 day after inoculating, the content of chlorophyll a and b in the leaves of susceptible varieties were lower than those of control, but the content of chlorophyll a and b in the leaves of resistant varieties were higher than control. Otherwise, the changes of chlorophyll a and b were differently effected by races of different pathogenicity, No.7 race of C.sojina was the strongest one to destroy structure of chloroplast,the second one was No.6 race, the weakest one was No.4 race, races No.2 and No.1 were in the middle. The specific values of chlorophyll a and b in leaves of resistant varieties were higher than those in susceptible varieties, we preliminarily considered that the specific value of chlorophyll a and b can be used as a biochemical marker for identification of soybean resistance to froge-eye leaf spot.
出处
《大豆科学》
CAS
CSCD
北大核心
2001年第1期49-53,共5页
Soybean Science
关键词
大豆灰斑病菌
叶绿素
品种
抗病性
大豆
Cercospora sojina Hara
Content of chlorophyll a and b
Resistant soybean varieties
Susceptible soybean varieties
