摘要
采用ＳＭＶ－ａ株系为毒源接种３个不同抗性类型的大豆品种（科丰１号，１１３８－２和徐豆１号），在接种后８，２４，４８，７２小时及在３，６，９，１２，１５，１８天共１１次连续取样与相应ＣＫ一并测定过氧化物酶的活性后发现：未经接种的抗感品种体内ＰＯ酶活性差异较大，感病品种中ＰＯ酶活性水平高于抗性品种２倍以上；接种后３天内抗性品种体内ＰＯ酶活性提高快，高峰值出现早，以接种后２４小时最为明显。接种后感病品种体内ＰＯ酶活性亦迅速提高，在接种后９天及１５天分别出现明显高峰。同时，叶片产生典型症状。结果认为ＳＭＶ侵染大豆后（尤其在接种后３天内）测定体内ＰＯ酶活性的变化。
Peroxidase activity were tested with leaves of resistant and susceptible soybean cultivars at 3 days interval up to 18 days and at 24 hours interval up to 72 hours. The results showed that the peroxidase activity in susceptile cultivars was higher than resistant without inoculation of SMV(CK). The resistant cultivars after inoculated with SMV-a showed an increase in peroxidase activity, the peak of peroxidase activity was at 24 hours after inocuilation with SMV. But the peroxidase activity increased greatly after 9 days and 15 days after inoculated with SMV-a. On the susceptible cultivars This results were coincided with symptoms development. The infected plants were symptomless on the resistant cultivar.
出处
《大豆科学》
CAS
CSCD
北大核心
1997年第1期28-33,共6页
Soybean Science
基金
国家自然科学基金
关键词
大豆
花叶病毒
过氧化物酶
Soybean mosaic virus
Peroxidase activity
