摘要
本文对大豆疫霉菌 ( Phytophthora sojae)的 1 5个菌株及其它 7种疫霉的 8个菌株进行了 4种同工酶电泳比较研究。结果表明 ,大豆疫霉菌种内菌株间 4种同工酶基本一致。疫霉属种间酯酶( EST)和超氧化物歧化酶 ( SOD)酶谱存在明显差异 ,表现为酶带的多少和 Rf值的不同 ;淀粉酶( DIA)和过氧化氢酶 ( CAT)同工酶酶谱差异不很明显 ,与某些疫霉种酶谱相似 ,只表现为酶带强度上的差异。本文首次报道了大豆疫霉菌的 SOD、CAT和 DIA同工酶 ,并指出 EST和 SOD同工酶在 P.sojae种的鉴定上具有重要意义 ,为 P.
Polyacrylamicide gel electrophoretic analysis of isozymes of 15 strains of P. sojae and 8 strains of seven other Phytophthora species was studied. The results show higher similarities between 15 strains from P.sojae in four isozyme zymograms. There are no marked differences of DIA and CAT zymograms between P.sojae and seven other Phytophthora species. Marked differences of EST and SOD zymograms among P.sojae and seven other Phytophthora species indicate that EST and SOD isozyme are useful and reliable supplementary tools for identification of P. sojae.
出处
《大豆科学》
CAS
CSCD
北大核心
2001年第1期1-4,共4页
Soybean Science
