摘要
对转 Bt基因水稻与常规品种的杂交后代进行了 GUS活性的组织化学分析和农艺性状考察。结果表明 ,报告基因 gus与抗虫基因 cry1Ab是连锁遗传和协同表达的 ,利用 GUS组织化学染色法可快速筛选抗虫杂种植株 ;无论是籼粳交 ,还是粳粳交后代 ,抗虫和感虫的两类植株间在株高、穗长、单株分蘖数、播始历期和千粒重等主要农艺性状上无显著差异 ,这无疑为利用
GUS activity was analyzed by histochemical staining and agronomic traits were observed in hybrid progenies between transgenic Bt rice and conventional rice varieties. Results showed that reporter gene gus was co inherited and co expressed with the insect resistant gene cry1Ab and the GUS histochemical assay can be used to rapidly screen insect resistant plants. And no significant differences in main agronomic traits, such as plant height, panicle length, the number of tillers per plant, days to heading and 1000 grain weight, were found between the two classes of the insect resistant plant and the susceptible plant in the progenies of either indica/japonica crosses or japonica/japonica crosses, providing scientific basis for utilization of transgenic Bt rice in cross breeding for elite varieties with insect resistance.
出处
《中国水稻科学》
CAS
CSCD
北大核心
2001年第2期101-106,共6页
Chinese Journal of Rice Science
基金
浙江省科委"九五"重大项目
美国洛氏基金资助项目!(RF970 0 1# 5 5 6)
