摘要
以较抗天牛的Ｉ－６９杨为母本，以Ｉ－６３杨、欧洲黑杨和晚花杨２７２为父本，于１９８３年春进行人工控制授粉，组合编号分别为３４、３２和３７。经苗期选择选出优良单株营造比较林，经５年生无性系比较林和９年生无性系比较林测定，采用主成分分析、主成分遗传距离聚类分析和选择指数，选出速生、抗虫和材质较好的纤维材无性系３４－３０１和３４－２８６。这两个新选出的无性系（３４－３０１和３４－２８６），在９年生时与对照Ｉ－６９杨相比材积分别提高２４．９％和１６．６％，抗虫性明显提高（单株幼虫数分别为０．４和０．５，而Ｉ－６９杨为５．３８）；与３４－３１４相比材积分别提高１６．７％和８．９％。化学物质测定表明，抗虫性与树皮中酚类含量和丹宁含量呈正相关。分子生物学测定表明，杨树对云斑天牛的抗性受基因控制，找到了与抗虫基因连锁的ＲＡＰＤ分子标记。
I
─69(Populus deltoides cl.‘Lux’(I─69/55),as a female parent plant,I─63(P. deltoides cl.
‘Harvard’(I─63/51))、P. nigra and P.× euramericana cv. ‘Serotina272’)as male parent
plants,artificially controlled pollination was conducted in 1983.Crosscombinations were
numbered as No. 34,No. 32 and No. 37 respectively. After selection inthe nursery and clone
test in Mian County of Shanxi Province from 1985 to 1993,2 superpoplar clones(34─301 and
34─286),Which grow fast,resistant to insect and with good quali-ty,were selected by using
method of principal components analysis,cluster analysis of genet-ic distance of principal
components and selection index. The volume of 34─301 and 34─286,which grow for 9 years,
were 24.9%and 16.6%higher than that of I─69 respectively,and16.7%and 8.9%higher than that
of 34─314 respectively. Resistance to Batocer horsfieldi of34─301 and 34─286 were
significantly higher than that of I─69,their population per tree were0.4 and 0.5,(for I─69 was 5.
38).There was apositive correlation between the resistance toB.h. and the content of tannins
and phenolics according to the analysis of chemical composi-tion. Resistance to B. h.was
controlled by genes according to the analysis of molecular biol-ogy(RAPD analysis ).Molecular
marker of RAPD (OPAD─01)was found,which linked togenes of resistance to B.h.
出处
《林业科学研究》
CSCD
北大核心
1995年第4期429-436,共8页
Forest Research
基金
国家"八五"攻关欧美杨胶合板材纸浆材新品种选育专题
关键词
杨树
无性系
育种
抗虫育种
云斑天牛
抗虫机制
poplar clone,breeding of pest resistance,Batocera horsfieldi,populationdynamics of
pest,mechanism of resistance
