摘要
目的:利用豚鼠脊髓匀浆建立Wistar大鼠实验性自身免疫性脑脊髓炎(EAE)的动物模型,为中枢神经系统脱髓鞘性疾病的研究提供实验依据。方法:以豚鼠全脊髓匀浆(GPSCH)与完全弗氏佐剂(CFA)的混合乳剂一次性给予大鼠足垫皮下注射,同时大鼠足背注射百日咳杆菌原液,建立EAE模型。结果:在免疫后12天开始出现肢体瘫痪,双后肢拖动等EAE临床症状。光镜下可见脑和脊髓组织中有大量炎性细胞浸润,炎性细胞聚集在血管周围呈典型的"袖套"样改变。结论:利用豚鼠脊髓匀浆制备Wistar大鼠EAE模型方法简单,稳定、可靠、发病率高。
Objective：To induce experimental autoimmune encephalomyelitis （EAE） in Wistar rat treated with guinea pig spinal cord homogenate （GPSCH）, and to provide the experimental value for the demyelinating disease of the central nervous system （CNS）. Method：Wistar rats were immunized with both GPSCH and CFA and Bordetelle pertussis ., the pathological changes of EAE were stud-ied with the aid of light microscopy .Results：The animal got the clinical symptom of EAE;the characteristic light microscopical findings are：perivascular inflammatory infiltration ,inflammatory cuffs of small blood vessels .Conclusion：The method of establishing the EAE model is stable , simple and of high incidence .
出处
《牡丹江医学院学报》
2014年第3期12-14,共3页
Journal of Mudanjiang Medical University
