摘要
目的:观察不同剂量阿托伐他汀对实验性自身免疫性脑脊髓炎(EAE)Wistar大鼠脊髓内核转录因子-κB(NF-κB)表达的影响,探讨阿托伐他汀对EAE发病保护的机制。方法:以豚鼠脊髓匀浆(GPSCH)诱发Wistar大鼠建立EAE模型,将实验动物分为正常组、EAE对照组、高剂量和低剂量阿托伐他汀治疗组。采用量化评分法比较不同剂量阿托伐他汀对EAE发病率、潜伏期、临床症状和组织学的影响,并采用免疫组织化学方法检测脊髓内NF-κB的表达。结果:与EAE对照组比较,高剂量阿托伐他汀治疗组EAE潜伏期延长(P<0.05),大鼠发病的严重程度减轻(P<0.05),NF-κB在脊髓中的表达降低(P<0.05);而低剂量阿托伐他汀治疗组EAE潜伏期、临床症状和NF-κB表达与EAE对照组相似,差异无统计学意义(P>0.05)。结论:高剂量阿托伐他汀对EAE大鼠有保护作用,其作用机制可能为高剂量阿托伐他汀对中枢神经系统的免疫机制有一定影响。
Objective To observe the effects of atorvastatin with different doses on the expression of nuclear factor-kappa B（NF-κB）in spinal cords of rats with experimental autoimmune encephalomyelitis（EAE） and to explore its protective mechanism.Methods The Wistar rats were divided into four groups in the experiment：control group,EAE control group,high dose and low dose atorvastatin treatment groups.The rat models of EAE were established by induction with guinea pig spinal cord homogenate.The histological changes were observed under light microscope,then the changes of incidence rate,latency,clinical symptoms and histology of EAE which were effected by atorvastatin with different doses were compared by method of quantization.And the expression of NF-κB was detected by immunohistochemistry.Results Compared with EAE control group,the latency in high dose atorvastatin treatment group was prolonged（P〈0.05）,the severity of disease in rats was reduced（P〈0.05）,the NF-κB expression in the spinal cord was decreased（P〈0.05）;but the latency,the clinical symptoms and the expression of NF-κB in low-dose atorvastatin treatment group were similar to EAE control group,the differences were not statistically significant（P〉0.05）.Conclusion High dose atorvastatin plays a protective role in rats with EAE and its mechanism may be involved in some impact on the immunological mechanism of the CNS.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2011年第4期607-611,773,共6页
Journal of Jilin University：Medicine Edition
基金
河北省卫生厅科研基金资助课题(20100473)
