摘要
目的研究岩黄连Corydalis saxicola的生物碱类成分及其抗氧化活性。方法应用多种柱色谱法进行分离纯化,根据化合物的波谱分析进行结构鉴定,采用DPPH自由基清除法进行抗氧化活性研究。结果从岩黄连全草70%乙醇提取物中共分离得到16个化合物,分别鉴定为卡维汀(1)、刺罂粟碱(2)、氢化小檗碱(3)、四氢巴马汀(4)、碎叶紫堇碱(5)、斯氏紫堇碱(6)、原阿片碱(7)、去氢碎叶紫堇碱(8)、脱氢异阿朴卡维汀(9)、小檗碱(10)、去氢分离木瓣树胺(11)、白屈菜红碱(12)、脱氢卡维汀(13)、黄堇碱(14)、异紫堇定碱(15)、深山黄堇碱(16)。体外抗氧化活性结果显示岩黄连生物碱对DPPH自由基清除作用较强,抗氧化活性显现浓度依赖性。结论化合物3、5、8、11、16均为首次从该植物中分离得到。化合物5、16抗氧化活性高,可能是该植物主要的抗炎活性成分。
Objective To study the alkaloids from Corydalis saxicola and their anti-oxidative activities.Methods The alkaloids were separated and purified by various column chromatography and identified according to their spectral analyses.The anti-oxidation activities were investigated on DPPH radical scavenging assay.Results Sixteen compounds were obtained and identified as cavidine（1）,stylopine（2）,canadine（3）,tetrahydropalmatine（4）,cheilanthifoline（5）,scoulerine（6）,protopine（7）,dehydrocheilanthifoline（8）,dehydroisoapocavidine（9）,berberine（10）,dehydrodiscretamine（11）,chelerythrine（12）,dehydrocavidine（13）,corypalline（14）,isocorydine（15）,and pallidine（16）.The alkaloids from C.saxicla were measured by the model of scavenging the stable DPPH radical,which showed a concentration dependent scavenging effect.Conclusion Compounds 3,5,8,11,and 16 are isolated from C.saxicola for the first time.Compounds 5 and 16 show the strong anti-oxidative activities.
出处
《中草药》
CAS
CSCD
北大核心
2014年第11期1526-1531,共6页
Chinese Traditional and Herbal Drugs
基金
广东省中医药科研项目(20111166)
关键词
岩黄连
生物碱
氢化小檗碱
碎叶紫堇碱
深山黄堇碱
抗氧化活性
Corydalis saxicola Bunting
alkaloids
canadine
cheilanthifoline
pallidine
anti-oxidative activities
