摘要
目的:探讨顺铂激活的低分化鼻咽癌细胞(CNE-2Z)氯通道电流是否为钙激活的氯电流。方法:采用膜片钳全细胞记录技术记录细胞内/外无Ca2+及钙通道阻断剂对顺铂激活氯电流的影响,并用高渗灌流液观察顺铂激活氯电流的容积敏感性。结果:去除细胞外液的Ca2+后,5μmol/L顺铂能诱发氯电流,且电流大小与细胞外有Ca2+时无明显差异,但潜伏期与达峰时间延长。细胞内外均无Ca2+对顺铂激活氯电流未产生影响。钙通道阻断剂nifedipine未能抑制顺铂诱发的氯电流。但细胞外灌流高渗液几乎可完全抑制顺铂激活的氯电流。结论:顺铂激活的氯通道开放不依赖于细胞内/外的Ca2+,该通道不是钙激活氯通道而很可能是容积敏感性氯通道。
AIM：To investigate the type of chloride channel activated by cisplatin in poorly differentiated na -sopharyngeal carcinoma cells （CNE-2Z cells）.METHODS：The technique of whole-cell patch-clamp was used to investigate the role of Ca ^2＋in the activation of cisplatin-activated chloride currents and to analyze the effect of hypertonic stress on these currents in CNE-2Z cells.RESULTS：Chloride currents were induced when the cells were exposed to the calcium -free cisplatin solution , showing the similar density to the currents induced by cisplatin with the presence of extracellular calcium.However , the latency and the peak time of cisplatin-activated currents in the absence of extracellular calcium were prolonged.The activation of cisplatin-activated chloride currents was insensitive to the depletion of intra-and extracellular calcium.Calcium channel antagonist nifedipine had no effect on the cisplatin -activated chloride currents , while hypertonic solution completely inhibited those currents .CONCLUSION：The cisplatin-activated chloride currents are independent on intra/extracellular calcium .The chloride channels activated by cisplatin are not calcium-activated chloride channels , but are probably volume-sensitive chloride channels .
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2014年第6期968-974,共7页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81173064
No.81272223
No.81273539)
教育部博士点基金资助项目(No.20124401110009)
广东省医学科学技术研究基金资助项目(No.B2012187)
广州市科技计划项目(No.2013J500015)
东莞市科技计划项目(No.2011108102006)
关键词
顺铂
鼻咽肿瘤
氯通道
钙
Cisplatin
Nasopharyngeal neoplasms
Chloride channels
Calcium
