摘要
目的:研究不同生长周期的鼻咽癌低分化上皮细胞(CNE-2Z)的迁移能力及氯通道在迁移过程中所起的作用。方法:运用血清饥饿法、化学药物双阻断法、有丝分裂药物阻断及摇落法分别将CNE-2Z细胞同步化至细胞周期的G1、S、M期,流式细胞仪检测其同步化效果。结合应用迁移小室和图像分析法,测定迁移率。台盼蓝活细胞染色法测定药物的细胞毒性。结果:不同生长周期的细胞迁移能力不同,G1期细胞迁移能力最强,随后是M期,S期迁移能力最弱。氯通道阻断剂(ATP、NPPB、tamoxifen)抑制CNE-2Z细胞迁移,但不同的氯通道阻断剂对不同生长周期CNE-2Z细胞迁移的阻滞效应不相同。结论:CNE-2Z细胞的迁移能力与其所在的生长周期密切相关,氯通道在各期CNE-2Z细胞的迁移过程中起着重要作用。
AIM： To clarify the migration capability of nasopharyngeal carcinoma cells （CNE - 2Z） at different stages of the cell cycle and the roles of chloride channels in cell migration. METHODS： Synchronous cells were obtained by the serum deprivation, double chemical - block, mitotic arrest and shake - off techniques. Cell cycle distribution of CNE - 2Z cells was analyzed by the flow cytometry. Migration rate was assayed by transwell chambers and by image analysis. The cytotoxicity of chemicals on cells was tested by MTT assay. RESULTS： CNE - 2Z cells at different stages of the cell cycle exhibited different migratory ability. The migration rate of the three stages was G1 〉 M 〉 S. The migration of CNE - 2Z cells was inhibited by chloride channel blockers （ATP, NPPB and tamoxifen）, but the inhibitory effect of the blockers varied with cells at different stages. CONCLUSIONS： The migratory ability is associated with the cell cycle in CNE - 2Z cells. Chloride channels play an important role in cell migration of CNE- 2Z cells.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2005年第9期1783-1786,共4页
Chinese Journal of Pathophysiology
基金
英国TheWellcomeTrust资助(No.056909/299/Z)
中国教育部资助(No.GJ9901)
广东省卫生厅资助项目(No.A2001474)
关键词
鼻咽肿瘤
细胞运动
氯通道
细胞周期
Nasopharyngeal neoplasms
Cell movement
Chloride channels
Cell cycle
