摘要
背景:碱性成纤维细胞生长因子是具有多功能的细胞生长因子,对来源于中胚层及神经外胚层的细胞有明显的促进增殖作用。目的:观察碱性成纤维细胞生长因子对体外培养人牙周膜细胞的作用。方法:将第5代人牙周膜细胞,以1×108 L-1的浓度分别接种到96孔板,随机分成4组,分别加入含0,1,10,100μg/L的碱性成纤维细胞生长因子、体积分数为15%胎牛血清的α-MEM培养基进行培养。在第1,3,5,7天测定细胞的增殖情况,在第1,7天检测碱性磷酸酶活性。结果与结论:4组之间人牙周膜细胞增殖情况的差异有显著性意义(F=6.586,P=0.024),随着碱性成纤维细胞生长因子质量浓度的增大,吸光度值均增大,其中100μg/L碱性成纤维细胞生长因子组的吸光度值均大于其他组(P<0.05);碱性成纤维细胞生长因子各组的碱性磷酸酶活性均低于对照组(P=0.000),浓度越大,活性越低(P<0.05)。结果显示碱性成纤维细胞生长因子在1-100μg/L范围内质量浓度越高,促进人牙周膜细胞增殖和抑制碱性磷酸酶活性的作用越强。
BACKGROUND：Basic fibroblast growth factor is a pluripotent cytokine that can promote the proliferation of mesodermal and neuroectodermal cells. OBJECTIVE：To observe the effect of basic fibroblast growth factor in human periodontal ligament cells cultured in vitro. METHODS：Human periodontal ligament cells at passage 5 were inoculated into the 96-wel plates at the density of 1×108/L, and were randomly divided into four groups. The cells were cultured inα-MEM containing 15%fetal bovine serum and 0, 1, 10, 100μg/L basic fibroblast growth factor, respectively. At 1, 3, 5, 7 days of the culture, the cellproliferation was determined, and the activity of alkaline phosphates was detected at 1 and 7 days. RESULTS AND CONCLUSION：There were significant differences in the proliferation of human periodontal ligament cells among the four groups （F=6.586, P=0.024）. As the increase of the basic fibroblast growth factor concentrations, the absorbance value was gradual y increased and reached the peak in 100μg/L basic fibroblast growth factor group （P〈0.05）. The alkaline phosphatase activity in basic fibroblast growth factor groups was lower than that of the control group （P=0.000）, the higher the concentration was, the lower activity was （P〈0.05）. Results show that basic fibroblast growth factor can promote the proliferation of human periodontal ligament cells and inhibit the activity of alkaline phosphatase, and the effect is concentration-dependent.
出处
《中国组织工程研究》
CAS
CSCD
2014年第20期3184-3189,共6页
Chinese Journal of Tissue Engineering Research
