摘要
目的探讨小檗碱(Berberine,BBR)对金黄色葡萄球菌(S.aureus)诱导人脐静脉内皮细胞株ECV-304凋亡的抑制作用。方法 BBR预处理ECV-304细胞2 h后,按细胞与细菌比例为1∶100感染复数的S.aureus感染细胞,MTT法检测细胞活性,采用Hoechst 33258染色观察细胞形态学变化,DNA Ladder检测BBR对感染细胞的保护作用。结果 ECV-304细胞活性受BBR影响不明显,S.aureus感染可显著降低ECV-304细胞活性(P<0.05),128μg/mL浓度的BBR可更好的保护细胞免受S.aureus的影响。用BBR预处理ECV-304后再感染S.aureus可抑制S.aureus诱导的细胞活性的降低(P<0.05),并减少细菌诱导的细胞凋亡,凋亡小体明显减少,并且DNA降解不明显。结论 BBR可以抑制S.aureus诱导的人脐静脉内皮细胞凋亡。
Objective To study the inhibition of berberine （BBR） against ECV-304 apoptosis in- duced by Staphylococcus aureus （S. aureus）. Methods ECV-304 cells were pre-treated with 128 μg/mL BBR for 2 h and then S. aureus was added （1： 100）. The viability of cells was detected by MIT （3-4,5- Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide） assay. The morphological changes were observed by Hoechst 33258 staining. The protection of BBR for infected cells was detected by DNA Ladder. Results ECV-304 cells＇ viability were not obviously affected by berberine. But S. aureus induced ECV-304 cells＇ vi- ability could be significantly inhibited by pre-treatment of BBR （P 〈 0.05）. Besides S. aureus-induced ECV-304 apoptosis could be reduced, with significantly lessened apoptotic body and unobvious DNA degradation. Conclusion BBR could significantly inhibit S. aureus induced ECV-304 apoptosis.
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2014年第6期710-713,共4页
Chinese Journal of Integrated Traditional and Western Medicine
基金
广东省中医药管理局科研课题资助项目(No.20121029)
关键词
小檗碱
金黄色葡萄球菌
人脐静脉内皮细胞
凋亡
berberine
Staphylococcus aureus
human umbilical vein endothelial cell
apoptosis
