摘要
OBJECTIVE： Few studies have investigated the ef- fects produced by combinations of polysaccharides and chemotherapeutic drugs in cancer treatment. We hypothesized that a combination of polysaccha- rides （COP） from Lentinus edodes and Tricholoma matsutake would improve the efficacy of 5-fluoro- uracil （5-FU）-mediated inhibition of H22 cell growth. METHODS： Mice were injected H22 cells and then treated with either 5-FU, polysaccharides from Tri- choloma matsutake （PTM）, polysaccharides from Lentinus edodes （PL）, PTM ＋ PL, 5-FU ＋ PTM, 5-FU ＋ PL, or 5-FU ＋ COR The tumor weight and volume, and splenic CD4 ＋ and CD8 ＋ T cell frequencies, were determined. Additionally, splenic natural killer （NK） cell and cytotoxic T lymphocyte （CTL） activities were assessed and the serum levels of tumor necro- sis factor-a （TNF-a）, Interleukin-2 （IL-2）, and Interfer- on-y （IFN-y） were measured. RESULTS： Compared with mice from the control,5-FU, PL, PTM, PTM ＋ PL, 5-FU ＋ PL, and 5-FU ＋ PTM groups, mice treated with 5-FU ＋ COP showed： （a） significantly reduced tumor weight and volume （P〈 0.05）, （b） significantly higher serum levels of TNF-α, IL-2, and IFN-y （P〈0.05）, （c） significantly increased CD4＋ and CD8＋ 1 cell frequencies in the spleen （P〈 0.05）, and （d） significantly increased splenic NK cell and CTL activities （P〈0.05）. The tumor weight and volume in mice treated with 5-FU＋PL or 5-FU＋PTM were significantly reduced compared with mice treated with 5-FU alone （P〈0.05）. Serum levels of TNF-α, 11.-2, and IFN-y, frequencies of CD4 ＋ and CD8＋ T cells in the spleen, and splenic NK and CTL activities were also significantly increased in mice treated with 5-FU＋PL or 5-FU＋PTM compared with mice treated with 5-FU alone （P〈0.05）. CONCLUSION： Polysaccharides from Lentinus edodes and Tricholoma matsutake could enhance the efficacy of 5-FU-mediated H22 cell growth inhi- bition.
OBJECTIVE: Few studies have investigated the effects produced by combinations of polysaccharides and chemotherapeutic drugs in cancer treatment.We hypothesized that a combinationo fpolysaccha rides(COP) from Lentinus edodes and Tricholoma matsutake would improve the efficacy of 5-fluorouracil(5-FU)-mediated inhibition of H22 cell growth.METHODS: Mice were injected H22 cells and then treated with either 5-FU, polysaccharides from Tricholoma matsutake(PTM), polysaccharides from Lentinus edodes(PL), PTM+PL, 5-FU+PTM, 5-FU+PL, or 5-FU+COP. The tumor weight and volume,and splenic CD4 + and CD8 + T cell frequencies,were determined. Additionally, splenic natural killer(NK) cell and cytotoxic Tlymphocyte (CTL) activities were assessed and the serum levels of tumor necrosis factor-α(TNF-α),Interleukin-2(IL-2),and Interferon-γ (IFN-γ) were measured. 5-FU, PL, PTM, PTM+PL, 5-FU+PL, and 5-FU+PTM groups, mice treated with 5-FU+COP showed:(a)significantly reduced tumor weight and volume(P<0.05);(b) significantly higher serum levels ofTNF-α,IL-2, and IFN-γ(P<0.05);(c) significantly increased CD4+andCD8+Tcell frequencies in the spleen(P<0.05); and(d) significantly increased splenic NK cell and CTL activities(P<0.05). The tumor weight and volume in mice treated with 5-FU+PL or 5-FU+PTM were significantly reduced compared with mice treated with 5-FU alone(P<0.05). Serum levels of TNF-α, IL-2, and IFN-γ, frequencies of CD4 + and CD8+T cells in the spleen, and splenic NK and CTL activities were also significantly increased in mice treated with 5-FU+PL or 5-FU+PTM compared with mice treated with 5-FU alone (P<0.05).CONCLUSION: Polysaccharides from Lentinus edodes and Tricholoma matsutake could enhance the efficacy of 5-FU-mediated H22 cell growth inhibition.
基金
Supported by Department of Science and Technology of Jilin Province(No.yyzx201123-2
No.20130522056JH)
