摘要
目的：探讨精液优化处理后孵育不同时间受精对体外受精-胚胎移植（in vitro fertilization-embryo transfer,IVF-ET）治疗的实验室指标及临床结局的影响。方法：选取2011年7月～2012年7月因女方输卵管因素导致不孕并行IVFET治疗的患者160例为研究对象。按受精时男方精液优化后孵育时间不同（1～2 h、2～3 h、3～4 h、4～6 h）而随机分为4组,每组40例。比较4组的MⅡ卵受精率、正常受精率、正常卵裂率、优质胚胎率、可用胚胎率、临床妊娠率、着床率、流产率。结果：分别比较4组患者的MⅡ卵受精率、正常受精率、正常卵裂率、优质胚胎率、可用胚胎率、临床妊娠率、着床率及流产率,差异均无统计学意义（P〉0.05）。结论：IVF-ET中精液优化后孵育6 h内受精并不影响实验室指标及临床结局。
Objective： To explore the effects of different tertilization times during semen incubation after optimization on laboratory indexes and clinical outcome of in vitro fertilization and embryo transfer （IVF -ET） . Methods： A total of 160 fertile patients because of tubal factors and treated with IVF -ET from July 2011 to July 2012 were selected as study objects; then the patients were divided into four groups according to different fertilization times （1 -2 hours, 2 -3 hours, 3 -4 hours, 4 -6 hours） during semen incubation after optimiza- tion, 40 patients in each group ; the fertilization rates of M ]I oocytes, the normal fertilization rates, the normal cleavage rates, the high quali- ty embryo rates, the embryo utilization rates, the clinical pregnancy rates, the implantation rates and the abortion rates in the four groups were compared. Results： There was no statistically significant difference in the fertilization rate of M ]I oocytes, the normal fertilization rate, the normal cleavage rate, the high quality embryo rate, the embryo utilization rate, the clinical pregnancy rate, the implantation rate and the abortion rate among the four groups （ P 〉 0. 05 ） . Conclusion ： Fertilization within six hours after sperm optimization during IVF - ET does not affect laboratory indexes and clinical outcome.
出处
《中国妇幼保健》
CAS
北大核心
2014年第19期3120-3123,共4页
Maternal and Child Health Care of China
关键词
精子孵育
体外受精
-胚胎移植
精液优化技术
临床结局
Sperm incubation
In vitro fertilization and embryo transfer
Semen optimization technology
Clinical outcome
