摘要
用高效液相色谱和放射免疫分析对长角血蜱Haemaphysalislongicornis若虫发育期整体蜕皮激素 (2 0 E)含量变化进行了测定 ,并用扫描电镜对其表皮结构及其发生进行了观察。 2 0 E存在于若虫整个发育期 ,饥饿、吸血和饱血后前 4天 ,激素水平低 (0 71～ 1 30ng/头 ) ;饱血后 6天明显增加 ,此时皮层溶离开始 ,蜕皮间隙形成 ;饱血后 7天继续增加 ;饱血后 8天 ,2 0 E含量达到高峰 (8 70ng/头 ) ,此时若虫开始沉积新的上表皮 ;高峰后 ,2 0 E含量急剧下降 ,直到蜕皮前保持低水平 ,此期内沉积新的原表皮 ,旧表皮被部分吸收。上述结果显示出 2 0 E含量急剧增加及其高峰分别与启动皮层溶离和新的上表皮沉积相吻合 ,2 0 E与新的原表皮沉积无关。
The changes of ecdysteroid (20\|E) in the whole body and cuticulogenesis during nymphal stage were studied by HPLC and RIA, and SEM respectively. 20\|E existed in all the nymphal stages. Its level was low in nymphs unfed, feeding and during the first 4 days after engorgement(0 71～1 30?ng/nymph). Then it began to increase sharply on the 6th day after engorgement when apolysis was initiated and exuvial space appeared. 20\|E reached its peak (8 70?ng/nymph) during the deposition of the new epicuticle on the 8th day after engorgement. Thereafter, 20\|E dropped to low value again. The procuticle deposition and old cuticle digestion occurred during this period. There results demonstrated that the highest concentration and sharp increase of 20\|E were coincident with the deposition of new epicuticle and the formation of exuvial space (apolysis) separately. No relationship was observed between 20\|E concentration and new procuticle synthesis.
出处
《昆虫学报》
CAS
CSCD
北大核心
2001年第1期46-50,共5页
Acta Entomologica Sinica
关键词
长解血蜱
若虫
20-羟基蜕皮酮
表皮发生
蜱
Haemaphysalis longicornis
nymph
20\|hydroxyecdysone
cuticulogenesis
