摘要
目的:优选信前胡的大孔树脂纯化工艺条件。方法:以白花前胡乙素提取量为评价指标,通过正交试验考察乙醇体积分数、溶剂用量、提取时间对信前胡提取工艺的影响。通过单因素试验筛选大孔树脂型号并考察大孔树脂吸附与洗脱条件。采用HPLC测定白花前胡乙素含量,流动相甲醇-水(75∶25),检测波长321 nm。结果:最佳提取工艺为加8倍量60%乙醇提取3.5 h。选择X-5型大孔树脂,吸附时间60 min,白花前胡乙素吸附率92.36%,洗脱率71.24%,提取率98.71%,纯度8.88%。结论:X-5型大孔树脂适合分离纯化白花前胡乙素,为信前胡香豆素类成分的开发应用提供参考。
Objective： To optimize purification process of praeruptorin B from Peucedani Radix by macroporous resin. Method： With extracting amount of praeruptorin B as index, orthogonal test was adopted to investigate effects of ethanol concentration, the amount of solvent and extracting time on extraction process. Type of macroporous resin and its elution conditions were investigated by single factor tests. HPLC was employed to determine the content of praeruptorin B with mobile phase of methanol-water （75： 25） and detection wavelength at 321 nm. Result： Optimum extraction technology was as following： extracted 3.5 h with 8 times the amount of 60% ethanol. X-5 macroporous resin was selected, its adsorption time was 60 min, adsorption rate, elution rate, recovery and purity were 92.36% , 71.24% , 98.71% and 8.88% , respectively. Conclusion： X-5 macroporous resin was suitable for separation and purification of praeruptorin B, this study could provide a reference for development and applicaiton of total coumarins from Peucedani Radix.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第13期35-37,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
深圳职业技术学院校级科研基金重点项目(601422K27001)
关键词
白花前胡乙素
大孔吸附树脂
前胡
总香豆素
praeruptorin B
macroporous resin
Peucedani Radix
total coumarins
