摘要
本文报道了采集广西产虎纹捕鸟蛛(Selenocosmia huwena)毒液的方法,并对粗毒进行了部分生物学活性的测定。该粗毒对小鼠和蜚蠊的LD_(50)分别为1.16mg/kg和300μg/g。该粗毒具有透明质酸酶、碱性磷酸酶、蛋白水解酶和脱氧核糖核酸酶活性。未测到磷脂酶A和胆碱脂酶活性。通过电生理实验发现该粗毒含有阻断蟾蜍神经肌肉接头传递的毒素,并观察到当小鼠接受腹腔注射致死剂量(5mg/kg)粗毒后便迅速出现呼吸麻痹。
A method of collect venom from the spider ( Selenocosmia huwena) was reported. The biological activities of the crude venom were analyzed. The LD50 of the venom in mice and cockroach were 1.16 mg / kg and 300μg / g respectively. It has enzyme activities of hyaluronidase, alkaline phosphatase, protease, and DNAse, but no choline esterase and phospholipase A were found in it. In the isolated toad sciatic nerve-sartorius preparation the venom at a concentration of 3mg / ml was found to block the neuromuscular transmission irreversibly. In mice, the crude venom in dosage of 5 mg / kg caused respiratory failure in about 20 seconds.
基金
国家自然科学基金
关键词
蜘蛛毒
虎纹捕鸟蛛
鉴定
Spider venom, Selenocosmia huwena, Neurotoxin, Hyalutonidase
