摘要
目的建立大鼠颈总动脉球囊损伤模型,观察损伤后动态组织病理学及P27的变化。方法应用PTCA球囊导管损伤大鼠颈总动脉,分别取术后即刻以及第7、14和28天损伤处及正常血管组织进行对照,行HE染色,观察损伤后不同时间点颈动脉的组织学变化,Western blot检测不同时间点蛋白P27的含量变化。结果建模成功率较高,达86.7%;球囊导管损伤使大鼠颈总动脉内膜剥脱和新生内膜增生,导致管腔狭窄;内皮细胞在第7天时有散在覆盖,第14天时内皮呈片状覆盖,第28天时完全覆盖;损伤后第7天,内膜已开始增生,第14-28天增生最快;P27蛋白在损伤后7 d含量最低,而后表现缓慢上升过程。结论大鼠颈总动脉球囊损伤模型成功建立,成功率显著提高;改良的方法建立颈总动脉球囊损伤模型可行,可为研究建模方法提供参考;球囊损伤后出现一系列的内膜细胞增生反应。
[Objective] To build rat carotid artery balloon injury model, and observe dynamic changes in histopathology and P27. [ Methods ] The percutaneous transiluminal coronary angioplasty （PTCA） balloon catheter was used to damage rat carotid artery,injury tissues immediately after surgery, 7 d, 14 d, 28 d and normal vascular tissue lesion were taken and compared, HE staining , histological changes in the carotid artery of different time points after injury were observed. P27 content changes at different time points were detected by Western blot analy- sis. [Results~ The success rate of modeling was 86.7%; balloon catheter injury in the rat carotid artery endarterec- tomy and neointimal hyperplasialed to stenosis; endothelial cells scattered in coverage when 7 d, 14 d flaky when endothelial cover, 28 d when completely covered; injury 7 d, intimal hyperplasia begins, 14 d to 28 d hyperplasia fastest; P27 protein seven days after the injury was the lowest, and then rose slowly during the performance. [ Con- clusion] Rat carotid artery balloon injury model was successfully established, the success rate was significantly im- proved; improved method to establish common carotid artery balloon injury model was feasible,which provides a refer- ence for the study of modeling methods; after balloon injury a series of endometrial cells proliferative responses occur.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2014年第13期13-17,共5页
China Journal of Modern Medicine
基金
江西省自然科学基金资助(No:20122BAB205027)
关键词
颈总动脉
球囊损伤
再狭窄
平滑肌细胞
P27
carotid artery
balloon catheter injury
restenosis
vascular smooth muscle cell
protein 27
