摘要
采用高效液相色谱法，对87份柿种质资源叶中的维生素C含量进行测定，分析柿种质资源叶维生素C含量的多样性，并对其中15份材料的叶维生素C含量年动态变化进行分析。结果表明：①15份野生和栽培资源叶中维生素C含量随生长时间呈多样性变化；柿近缘种、野柿、甜柿、涩柿品种叶中维生素C含量的变异系数分别为33．21％、18．61％、30．89％、25．30％，含量均值为13．58、10．13、9．98和9．21mg／g；野生资源叶中的维生素C含量从展叶期到落叶期一直保持较高水平。②87份柿种质资源叶中的维生素C含量为1．93～23．91mg／g，变异系数为44．17％，多样性指数为1．96；野生资源类型的叶中维生素C含量极显著高于甜、涩柿品种类型（P〈0．01），而甜柿类型和涩柿类型叶中维生素C含量间的差异无统计学意义；聚类分析结果表明，按柿叶维生素C含量的高低可将柿种质资源分为高、中、低3个类群，其中类群Ⅲ的10份资源（湖南野柿1号、美洲柿、浙江柿、牛头柿、南化牛心柿、江苏野柿2号、湖北野柿2号、大平核、孟津红柿和湖北野柿1号）为柿叶维生素C含量高的种质资源。
Diversity and content of vitamin C in persimmon germplasm were analyzed using RP-HPLC by taken 87 persimmon germplasm leaves as resources, and 15 out of them were employed to their annual variation research. The results indicated that the vitamin C content in leaves of 15 wild and cultivar genotypes had a complex variation with growing time; the variation coefficient of vitamin C in the related varieties, silvestris persimmon, non-astringent varieties and astringent varieties were 33.21%, 18.61%, 30.89%, and 25.30% respectively, with their respective mean of 13.58, 10.13, 9.98, and 9.21 mg/g; vitamin C content in wild genotypes maintained a higher level from shoots expanding to leave falling period. Content of vitamin C in the 87 persimmon germplasm varied from 1.93 to 23.91 mg/g, with a variation coefficient of 44.17% and a diversity index of 1.96; vitamin C content in wild genotypes was significantly （P 〈 0.01） higher than that of non-astringent varieties and astringent varieties, whilst, there was no statistical difference between non-astringent varieties and astringent varieties. The persimmon germplasm could be classified into three types （high, medium and low） according to the level of vitamin C content from the result of cluster analysis. Out of 10 gerrnplasm in groups Ⅲ （No.1 of Hunanyeshi, D. viriginiana, D. glaucifolia, Niutoushi, Nanhuaniuxinshi, No.2 of Jiangsuyeshi, No.2 of Hubeiyeshi, Dapinghe, Mengjinhongshi and No. 1 of Hubeiyeshi） were rich in vitamin C.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2014年第3期288-293,共6页
Journal of Hunan Agricultural University(Natural Sciences)
基金
国家"十二.五"农村领域科技计划项目(2013BAD14B05)
关键词
柿
叶片
维生素C
年动态变化
多样性
persimmon
leaves
vitamin C
annual variation
diversity
