摘要
目的：研究复方龙葵颗粒对四氯化碳（CCl4）致大鼠慢性肝损伤的防治作用,并探讨其可能的作用机理。方法：大鼠60只随机分成6组：空白组、模型组、联苯双酯组、龙葵颗粒大剂量组（10g生药/kg）、中剂量组（5g生药/kg）、小剂量组（2.5g生药/kg）。除正常对照组外,均开始给予25%的CCl4花生油溶液3 ml/kg,2次/周,1次皮下注射和1次灌胃交替造模,连续10周。造模三周后各治疗组给予不同剂量复方龙葵颗粒干预,阳性对照组给予联苯双酯干预,腹主动脉取血,检测各组大鼠血清的ALT、AST、ALP、T-bil、TP、ALB含量,计算A/G比值;检测大鼠血清中SOD、GSH-Px的活性和MDA含量;解剖取大鼠肝脏、脾、胸腺称重,计算脏器指数,测定肝组织羟脯氨酸（Hyp）含量,并对肝组织进行病理组织学检查。结果：与模型组比较,复方龙葵颗粒中（5g生药/kg）、大剂量组（10g生药/kg）可显著降低CCl4所致大鼠慢性肝损伤血清中ALT、AST、ALP、T-bil水平,升高血清中TP,ALB含量;升高血清中SOD、GSH-Px的活性（P〈0.05~0.01）,并降低MDA的水平;肝组织内Hyp明显降低;病理观察结果可明显减轻慢性肝损伤的肝脏损伤程度。结论：复方龙葵颗粒对CCl4所致大鼠慢性肝损伤有明显的保肝作用,其机制可能与抑制脂质过氧化和清除体内过多的氧自由基的作用,并且提高机体的免疫力有关。
Objective： To study the prevention and therapeutic effect of complex granula of Solanum nigrum on chronic liver injury by carbon tetrachloride （ CCI4 ） in rats and explore its possible mechanism. Methods： 60 Spragne-Dawley rats were divided into 6 groups,including normal control group,model group, bffendate contrast group and Solanum nigrum granule high（ 10 g/kg）, medium（5 g/kg）, 1ow（2.5 g/kg） dose group. All rats except normal control group were given 25% carbon tctrachloride （3m]/kg） twice a week for 10 weeks, once subcutaneously injected once administered. After 3 weeks of the beginning,the rats of Solanum nigrum granule high, medium, low dose group were administered with Solanum nigrum granule at a dose of 10 g/kg body weight, 5 g/kg and 2.5 g/kg for 7 weeks. The rats of bifcndate contrast group were administered with bifendate at a dose orS0 mg/kg. The liver function（ ALT,AST,ALP,T-bil,TP,ALB in serum） was detected, and A/ G was calculated. The activity of SOD, GSH-Px , and the level of MDA in serum and levels of Hyp in liver tissue were measured. The organ index of liver, spleen and thoracic gland were detected. The histo-pathology of liver was examined. Result： Compared with model group, complex granula of Solanum nigrum high（ 10 g/kg）, medium（5 g/kg） dose group was found to significantly decrease the levels of ALT,AST, ALP and T-bil in serum （ P 〈0.05 -0.01 ）. And the level of ALB and TP were increasd （ P 〈 0.05 - 0.01 ）. Meanwhile it was also decreased MDA content （ P 〈 0.01 ） and prevented the reduction of SOD, GSH-Px activities in serum （ P 〈 0.05 - 0.01 ）. The level of Hyp in liver homogenate was decreased（ P 〈 0.05 - 0.01 ）. And it could also improve the pathological changes in liver tissue. Conclusions： These results suggested that complex granula of Solanum nigrum has significantly effect on chronic liver injury induced by CCl4 in rats. The protective effect may be related to its antioxidant activity. It could rid of excess oxygen free radicals, and improve the immunity of rats.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2014年第1期105-109,共5页
Pharmacology and Clinics of Chinese Materia Medica
基金
江苏省科技基础设施建设计划专项项目(BM2009903)
关键词
复方龙葵颗粒
四氯化碳
慢性肝损伤
保肝
complex granula of Solanum nigrum（复方龙葵颗粒）
carbon tetrachloride
chronic liver injury
hepatoproteetive effect
