摘要
目的:以昆明种小鼠为研究对象,研究雄黄对实验动物肝脏功能的影响,为其临床合理应用提供依据。方法:不同剂量雄黄(0.1、0.2、0.4 g/kg)灌胃3周,HE染色、观察肝细胞变化并称重,计算肝脏系数,紫外分光光度法测定血清中ALT、AST、ALP和γ-GT的活性、肝细胞膜ATP酶活性,流式细胞仪检测肝细胞周期的变化。结果:给各药组肝脏系数明显降低,肝细胞受损伤程度与给药剂量正相关;且小鼠肝功能出现明显变化,ALT、AST、ALP、γ-GT活性明显升高,ATPase活力降低,肝细胞周期阻滞在S期和G2/M期。结论:雄黄长期给药抑制小鼠体重,影响肝功能,损伤肝细胞。
Objective： To study the realgar inducing liver injury by intragastric administration and reveal the mechanism of realgar inducing liver injury. Methods： Realgar dose were 0. 1g/kg,0.2g/kg,0.4g/kg, kunming mice as experimental animals for 3 weeks. The effect of realgar on liver coefficient was made histological observation, the effects of enzyme activities of serum alanine aminotransferase （ALT）, aspartate amin- otransferase （AST）, alkaline phosphatase （ALP） ,7-glutamyl transpeptidase （7-GT） and liver cell membrane ATP were determined by ultraviolet spectrophotometry. The effect of Realgar on mice liver cell cycle was determined by flow cytometry. Results： The results showed that liver coefficient increased significantly. Histological observation showed realgar could cause swelling, degeneration, necrosis and preliferationof mice liver cell. Activities of ALT, AST, ALP and γ- GT had obvious increasing,the activity of ATP was decreased. Flow eytometry resuits showed Realgar made the percentage of S and G2/M phase increase significantly. Conclusion： Realgar can injury liver cells.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2014年第1期56-58,共3页
Pharmacology and Clinics of Chinese Materia Medica
关键词
雄黄
肝脏系数
肝功能
细胞周期
肝损伤
realgar（雄黄）
liver coefficient
liver function
cell cycle
liver injury
