摘要
目的:研究健脾活血方对大鼠急性酒精性肝损伤的防护作用。方法:将36只SD大鼠随机分为正常组、急性酒精性肝损伤模型组和中药干预组。模型组和中药干预组每日白酒灌胃连续5天,复制大鼠急性酒精性肝损伤模型。中药干预组予健脾活血方灌胃。实验结束处死大鼠,收集血清和肝脏组织,检测血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、肝组织γ-谷氨酰转肽酶(γ-GT)活性和肝组织甘油三酯(TG)含量。肝组织行HE染色和油红O染色,检测肝脏病理改变。结果:健脾活血方显著降低大鼠肝脏/体重比,降低血清ALT、AST和肝组织γ-GT活性,减少肝组织TG含量,明显减轻模型大鼠肝内脂肪沉积,改善肝细胞的脂肪性病理改变。结论:健脾活血方干预对大鼠急性酒精性肝损伤有较好的防护作用。
Objective： To study the effect of Jianpi Huoxue decoction on acute alcoholic liver injury in rats. Methods： Thirty-six Sprague Dawley rats were allocated to the control group, acute alcoholic liver injury model group and Jianpi Huoxue decoction group. Rats in model and Jianpi Huoxe decoction groups were administered with alcohol by gastric perfusion for 5 days to reproduce the model of acute alcoholic liver injury. Jianpi Huoxue decoction was administered by gastric perfusion. At the end of experiments, rats were sacrificed to collect the blood and liver samples. Activities of serum alaline aminotransferase （ ALT）, aspartate aminotransferase （AST） and hepatic gamma-glutamyltransferase （γ-GT）, and amount of hepatic triglycerides （TG） were determined. Hemotoxin and Eosin staining and oil red O staining were performed to observe pathological changes in liver samples. Results： Jianpi Huoxue decoction decreased the ratio of liver/body weight, reduced activities of serum ALT, AST and hepatic γ-GT, decreased hepatic TG amount significantly; alleviate the deposition of fatty in liver, ameliorate the pathological change of hepatocytes significantly. Conclusion： Jianpi Huoxue decoction has good prevention effect on acute alcoholic liver injury in rats.
出处
《中西医结合肝病杂志》
CAS
2009年第4期221-223,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases
基金
上海市科委启明星计划(No.07QA14052)
上海市教委重点学科建设项目(No.Y0302)
关键词
健脾活血方
急性酒精性肝损伤
大鼠
Jianpi Huoxue decoction
acute alcoholic liver injury
rat
