摘要
从水稻中分离获得的一个新的、功能未知的MYB基因,序列分析发现是一个典型的R2R3-MYB转录因子,与拟南芥中的MYB106高度同源,因此命名为OsMYB106。亚细胞定位检测结果表明,OsMYB106蛋白定位于细胞核中。Real Time PCR结果显示OsMYB106在水稻幼穗时期表达最高,并且受干旱、盐处理和冷胁迫的显著诱导上升表达。OsMYB106RNAi抑制植株表现出矮化以及花粉败育。基因表达分析显示花粉发育相关基因OsMS2和CYP704B2在抑制植株中表达显著降低,揭示OsMYB106可能是通过调控OsMS2和CYP704B2介导花粉细胞壁的发育进而影响花粉的育性。
A novel unknown MYB gene,designated OsMYB106,was isolated from rice,which was a typical R2R3-MYB transcription factor, and homologous to MYB106 in Arabidopsis. Subcellular localization indicated that OsMYB106 protein was located in nucleus. Real Time PCR showed that OsMYB106 was expressed highly in young panicle, and induced obviously by drought and cold stress. MYB106 RNAi plant showed dwarf and sterility. Further,pollen development related gene OsMS2 and CYPTO4B2 were down-regulated notably in plants MYB106 RNAi, the results indicated that OsMYB106 regulated OsMS2 and CYPTO4B2 involving in development of pollen cell wall to control the fertility of pollen.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2014年第4期7-12,共6页
Journal of Huazhong Agricultural University
基金
国家自然科学基金青年项目(31201091)
湖北省自然科学基金项目(2012FFB07408)
