摘要
目的 探讨 T- 2毒素与软骨细胞凋亡的关系。方法 水囊引产胎儿的软骨细胞体外单层培养。 T- 2毒素浓度分为 0 ,5 ,10 ,2 0和 40μg/ L 5组 ,第 1代软骨细胞接种后培养 4d,加入不同浓度的 T- 2毒素 ,作用 16 h。应用 TU NEL 法和流式细胞技术定性和定量检测软骨细胞凋亡。同时观察 T- 2毒素对软骨细胞增殖的影响。结果 各组细胞在加入 T- 2毒素后 ,胞体明显皱缩 ,T- 2毒素浓度越大 ,皱缩越严重。流式细胞仪检测与 Td T中介脱氧尿苷三磷酸末端标记 (TU NEL)法检测均发现 ,当 T- 2毒素在 0～ 10 μg/ L 之间时 ,T- 2毒素浓度越大 ,凋亡细胞越多。结论 T- 2毒素对软骨细胞的增殖有明显的抑制作用 ,并与 T- 2毒素浓度呈剂量—效应关系。 T- 2毒素可以引起体外培养的软骨细胞凋亡 ,且与 T- 2毒素浓度有一定关系。
Objective To investigate the effect of T 2 toxin on apoptosis of chondrocytes.Methods Chondrocytes which were obtained from aborted fetal were cultured in vitro.Four days later,these chondrocytes were exposed to T 2 toxin in different concetrations for 16 hours.According to the concentrations,five experimental groups were divided:0,5,10,20,40 μg/L.Then TUNEL staining and Flowcytometry were used to detect the apoptosis of chondrocytes qualitatively and quantitatively,the effect of T 2 toxin on proliferation of chondrocytes were also observed.Results After being exposed to T 2 toxin,the body of chondrocytes shrinked obviously and there was a dose dependent relationship between the toxin concentration and the degree of shrink.The concentration of T 2 toxin changed from 0 μg/L to 10 ng/ml,the number of apoptosis increased.Conclusions T 2 toxin can inhibit the proliferation of chondroyte significantly in a dose depenent manner. T 2 toxin can induce the apoptosis of chondrocyte and the numbers of apoptosis is proportionate to the concentration of T 2 toxin in particular range.
出处
《中国地方病学杂志》
CAS
CSCD
北大核心
2001年第2期84-86,共3页
Chinese Jouranl of Endemiology
