摘要
目的研究整合子参与铜绿假单胞菌耐药的分子机制。方法收集2008年1月至2011年12月我院临床分离的200株铜绿假单胞菌,采用K-B法进行体外药敏试验,采用聚合酶链式反应进行整合子整合酶基因的检测;整合子可变区扩增、克隆、测序,分析整合子基因结构。结果 59.0%的医院感染铜绿假单胞菌I类整合子阳性,未检测出II、Ⅲ类整合子;编码对氨基糖苷类、磺胺类抗菌药物和氯霉素耐药的基因整合子阳性组多药耐药菌均明显高于阴性组。结论 I类整合子在医院感染铜绿假单胞菌中广泛分布,可通过质粒在不同菌属间水平传播,在耐药基因传播中起重要作用,应引起临床足够的重视。
Objective To study the molecular mechanism of integron mediated resistance in Pseudomonas aeruginosa. Methods 200 strains were collected from January 2008 to December 2011. All strains were tested by K-B method for drug resistance. PCR and DNA sequencing method were used to detect integron and clarify the context of gene cassette. Results 59.0 % of strains were shown to be positive for class iintegron, and no class II and III integrons were detected. The multi-drug resistance in positive gene integron group were significantly higher than that in negative group, which coding for aminoglycosides, sulfonamides and chloramphenicol resistance. Conclusion Class I integron was widespread in Pseudomonas aeruginosa isolates which are causing nosocomial infection. Class I integrons in Pseudomonas aeruginosa infection in hospitals is widely distributed, transmitted in different pieces strains by plasmid, and playing an important role in the spread of resistance genes, therefor, thus should be paid sufficient attention.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2014年第6期462-465,共4页
Chinese Journal of Antibiotics
关键词
铜绿假单胞菌
整合子
耐药
Pseudomonas aeruginosa
Integron
Antibiotic resistance
