摘要
目的研究肺炎克雷伯菌的整合子流行现状,探讨其与耐药性的相关性。方法常规方法收集肺炎克雷伯菌,用K-B法测定其对13种常用抗菌药物的耐药性;采用PCR扩增肺炎克雷伯菌整合子基因,产物用琼脂糖凝胶电泳法检测,判断整合子基因与敏感耐药之间的关系。结果 115株肺炎克雷伯菌检出整合子49株,检出率42.6%;整合子阳性的菌株耐药性较高,特别是对阿米卡星、头孢他啶、头孢噻肟、头孢呋辛、哌拉西林、哌拉西林/他唑巴坦、磺胺甲噁唑/甲氧苄啶及左氧氟沙星等8种抗菌药物的耐药较为明显。结论肺炎克雷伯菌的耐药性与整合子基因密切相关,整合子对细菌的耐药起着重要作用。
OBJECTIVE To study integron prevalent status of Klebsiella pneumoniae and the relevance to drug-resistance. METHODS The clinical isolates of K.pneumoniae were collected.Drug resistance was determined by K-B disk diffusion method.K.pneumoniae integron gene was amplified by polymerase chain reaction（PCR）;Products were detected by agar gel electrophoresis.The relationship between the existence of integron genes and their drug-resistance was analyzed finally. RESULTS Among 115 strains of K.pneumoniae,integron gene were found in 42.6%（49/115）.The gene-positive strains showed higher drug resistance,especially in mikacin,ceftazidime,cefotaxime,cefuroxime,piperacillin,piperacillin/tazobactam,trimethoprim/sulfamethoxazole and levofloxacin antibiotic resistance. CONCLUSION There is tightly relationship between the existence of integron genes and drug resistance;integron plays an important role in drug resistance.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第22期4665-4667,共3页
Chinese Journal of Nosocomiology
基金
南京市卫生局科研项目(ykk06103)
关键词
肺炎克雷伯菌
耐药性
整合子
Klebsiella pneumonia
Drug resistance
Integron
