摘要
目的:探讨二至丸对乙醇诱导的大鼠急性肝损伤的保护作用及其作用机制。方法:雄性大鼠72只随机分成6组,每组12只,分别为正常对照组,模型组,二至丸低、中、高剂量组(3.78,7.56,15.12 g·kg-1),阳性对照凯西莱60 mg·kg-1组。模型组以56%二锅头酒ig给药10 d,后开始给药,连续7 d,采用乙醇灌胃法建立大鼠急性乙醇性肝损伤模型,检测血清丙氨酸转氨酶(ALT)、天冬氨酸转氨酶(AST)、乙醇脱氢酶(ADH)、甘油三酯(TG)活性和肝匀浆超氧化物歧化酶(SOD)、丙二醛(MDA)含量,观察肝脏病理形态学改变。结果:与模型组比较二至丸各剂量组均能显著降低血清ALT,AST,ADH水平;二至丸的高、中剂量组能显著降低肝匀浆中MDA含量,提高SOD活力;高剂量组可显著降低TG活性;通过病理学切片观察,二至丸各剂量组均能显著改善肝组织的病理变化,以二至丸高剂量效果最好。结论:二至丸对大鼠急性乙醇性肝损伤具有保护作用。
Objective： To investigate the protective effect of Erzhi pill on acute liver injure of rats induced by alcohol and its mechanism. Method： By using the alcohol induced acute liver injury rat model, the effects of Erzhi pill on the levels of alanine aminotransferase （ALT） , aspartate aminotransferase （AST） , alcohol dehydrogenase （ADH） and triglycerides （TG） in serum and the contents of superoxide dismutase （SOD）, malondialdehyde （MDA） in liver homogenate, and the pathological change of liver tissue were observed. Result： The Erzhi pill significantly decreased the levels of ALT, AST, ADH in serum ; the high dosage group decreased the content of MDA in liver homogenate, and increased the activity of SOD ; only the high dosage group decreased the levels of TG ; the histopathological analysis suggested that all of the treatment groups could significantly ameliorate pathological changes of hepatocytes, among the total, the high dosage group showed best. Conclusion： Erzhi pill has protective effect on acute liver injury of rats induced by alcohol.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第12期153-156,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81001599)
教育部博士点新教师基金项目(20103237120010)
关键词
二至丸
乙醇
急性肝损伤
Erzhi pill
alcohol
acute liver injury
