摘要
目的：研究莪术醇在正常孕大鼠及血瘀证孕大鼠体内的药动学差异，建立一个快速、灵敏的高效液相色谱测定大鼠血浆中莪术醇的分析方法。方法：Wistar雌性大鼠随机平均分为血瘀证组和正常组；采用皮下注射盐酸肾上腺素及冰水浴复制大鼠血瘀证模型。造模成功后受孕，尾静脉注射莪术醇药液，采用HPLC法对大鼠的血浆中莪术醇的浓度进行监测。结果：正常孕大鼠和血瘀证孕大鼠尾静脉注射莪术醇后，药动学参数为：V_c分别为0．570，0．569L·kg-1；CL分别为5．78，6．38mL·min-1；K分别为1．0，1．239h；t1／2分别为0．693，0．564h；AUC㈦分别为0．577，0．522mg·h-1·L-1；各时间点血药浓度存在差异（P〈0．05）。结论：静注给药后莪术醇在大鼠体内表现为快速分布和消除，莪术醇在正常孕大鼠和血瘀证孕大鼠血中的药物浓度具有差异。
Objective ： This study aims at evaluating the differences of pharmacokinetic behavior between normal and biood-stasis pregnant rats after intravenous administration and developing a rapid and sensitive high performance liquid chromatography method for the determination of curcumol in rat plasma. Method： Wistar female rats were randomized into normal group and blood-stasis model group. A model of blood-stasis model in rats by injecting adrenalin and being immersed in ice water was used. Then the rats were conceived and intravenous injected curcumol. The HPLC method was used to monitor the curcumol concentration in rat plasma. Result： There was a difference of plasma concentration after intravenous administration curcumol between normal and bloodstasis pregnant rats （P 〈0. 05） The value of Vc were 0. 570, 0. 569 L ·kg-1 ; CL were 5.78 6.38 mL · min-1 Ke were 1.0, 1. 239 h; tl/2 were 0. 693, 0. 564 h; AUC0-4 were 0. 577, 0. 522 mg -h-1· L-1 respectively. The plasma coneentrationswere different at each time point. Conclusion： The rapid distribution and elimination are performed after intravenous administration cureumol in rats. The curcumol concentration is different between normal and blood-stasis pregnant rats.
出处
《中国实验方剂学杂志》
CAS
北大核心
2014年第12期124-127,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81273640)
中国博士后科学基金项目(132930)
关键词
莪术醇
高效液相色谱
血瘀证
药动学
curcumol
HPLC
syndrome of blood stasis
pharmacokinetics
